Human squamous cell carcinoma antigen (SCCAg) ELISA Kit | MyBioSource MBS263873 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human squamous cell carcinoma antigen (SCCAg) ELISA Kit

catalog :

MBS263873

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS263873

products type :

ELISA Kit

products full name :

Human squamous cell carcinoma antigen (SCCAg) ELISA Kit

products short name :

squamous cell carcinoma antigen (SCCAg)

other names :

squamous cell carcinoma antigen recognized by T-cells 3; Squamous cell carcinoma antigen recognized by T-cells 3; squamous cell carcinoma antigen recognized by T-cells 3; SART-3; hSART-3; tat-interacting protein of 110 kDa; squamous cell carcinoma antigen recognized by T cells 3; Tat-interacting protein of 110 kDa; Tip110

products gene name :

SCCAg

other gene names :

SART3; SART3; P100; p110; DSAP1; TIP110; p110(nrb); RP11-13G14; KIAA0156; TIP110; SART-3; hSART-3; Tip110

uniprot entry name :

SART3_HUMAN

reactivity :

Human

sequence length :

963

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant SCCAg concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay: <= 12%

products description :

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human SCCAg monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

7661952

ncbi acc num :

NP_055521.1

ncbi gb acc num :

NM_014706.3

ncbi mol weight :

13,773 Da

ncbi summary :

The protein encoded by this gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing. [provided by RefSeq, Jul 2008]

uniprot summary :

SART3: a ubiquitously expressed protein in proliferating cells. A component of the 7SK snRNP complex composed of P-TEFb (composed of CDK9 and CCNT1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Transiently associates with the U6 and U4/U6 snRNPs during the spliceosome cycle, helping to regenerate functional U4/U6 snRNPs complexes. Expressed in the nucleus of all of malignant tumor cell lines tested and the majority of cancer tissues with various histologies, including squamous cell carcinomas, adenocarcinomas, melanomas and leukemias. However, this protein is undetectable in the nucleus of nonmalignant cell lines or normal tissues, except for the testis. Expressed in the cytoplasm of all proliferating cells, including normal and malignant cells, but not in most cells of normal tissues, except for the testis and the fetal liver. Regulates Tat transactivation activity through direct interaction. May be a cellular factor for HIV-1 gene expression and viral replication. 3 isoforms of the human protein are produced by alternative splicing. Protein type: RNA-binding; Transcription regulation; Nuclear receptor co-regulator. Chromosomal Location of Human Ortholog: 12q24.1. Cellular Component: nucleoplasm; Cajal body; U4/U6 x U5 tri-snRNP complex; cytoplasm; nuclear speck; nucleus. Molecular Function: U4 snRNA binding; protein binding; U6atac snRNA binding; histone binding; nucleotide binding; U6 snRNA binding. Biological Process: assembly of spliceosomal tri-snRNP; nuclear mRNA splicing, via spliceosome; nucleosome assembly; spliceosomal snRNP biogenesis; regulation of gene expression; cell morphogenesis; homeostasis of number of cells. Disease: Porokeratosis 3, Disseminated Superficial Actinic Type

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

420

more info or order :

MyBioSource product webpage