Mouse antiglomerular basement membrane (GBM) ELISA Kit | MyBioSource MBS263871 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse antiglomerular basement membrane (GBM) ELISA Kit

catalog :

MBS263871

quantity :

48-Strip-Wells

price :

260 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS263871

products type :

ELISA Kit

products full name :

Mouse antiglomerular basement membrane (GBM) ELISA Kit

products short name :

[antiglomerular basement membrane (GBM)]

other names :

[glutamate--cysteine ligase regulatory subunit; Glutamate--cysteine ligase regulatory subunit; glutamate--cysteine ligase regulatory subunit; GCS light chain; GSC light chain; gamma-ECS regulatory subunit; glutamate--cysteine ligase modifier subunit; glutamate-cysteine ligase regulatory protein; gamma-glutamylcysteine synthetase regulatory subunit; glutamate-cysteine ligase modifier subunit delta2 alternative splicing; glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory (30.8kD); glutamate-cysteine ligase, modifier subunit; GCS light chain; Gamma-ECS regulatory subunit; Gamma-glutamylcysteine synthetase regulatory subunit; Glutamate--cysteine ligase modifier subunit]

products gene name :

[GBM]

other gene names :

[GCLM; GCLM; GLCLR; GLCLR]

uniprot entry name :

GSH0_HUMAN

reactivity :

Mouse

sequence length :

274

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Mouse serum, plasma or cell culture supernatant. Detection Range: 100 ng/ml-1.56 ng/ml. Sensitivity: 0.5 ng/mL

other info2 :

Intra-assay Precision: ? 8%. Inter-assay Precision: ? 12%

products description :

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse GBM monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

4504011

ncbi acc num :

NP_002052.1

ncbi gb acc num :

NM_002061.2

ncbi mol weight :

30,727 Da

ncbi pathways :

Biological Oxidations Pathway (105698); Glutathione Biosynthesis, Glutamate = Glutathione Pathway (413393); Glutathione Biosynthesis, Glutamate = Glutathione Pathway (468307); Glutathione Conjugation Pathway (105727); Glutathione Metabolism Pathway (82973); Glutathione Metabolism Pathway (198824); Glutathione Metabolism Pathway (343); Glutathione Synthesis And Recycling Pathway (105728); Keap1-Nrf2 Pathway (198777); Metabolic Pathways (132956)

ncbi summary :

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq, Jul 2008]

uniprot summary :

GCLM: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq, Jul 2008]. Protein type: Other Amino Acids Metabolism - glutathione. Chromosomal Location of Human Ortholog: 1p22.1. Cellular Component: glutamate-cysteine ligase complex; cytosol. Molecular Function: protein heterodimerization activity; glutamate-cysteine ligase activity; enzyme regulator activity; glutamate-cysteine ligase catalytic subunit binding. Biological Process: response to drug; glutamate metabolic process; response to nitrosative stress; apoptotic mitochondrial changes; cysteine metabolic process; sulfur amino acid metabolic process; xenobiotic metabolic process; glutathione biosynthetic process; response to oxidative stress; negative regulation of neuron apoptosis; regulation of mitochondrial depolarization; regulation of blood vessel size; positive regulation of glutamate-cysteine ligase activity. Disease: Myocardial Infarction, Susceptibility To

size1 :

48-Strip-Wells

price1 :

260 USD

size2 :

96-Strip-Wells

price2 :

405

size3 :

5x96-Strip-Wells

price3 :

1615

size4 :

10x96-Strip-Wells

price4 :

2880

more info or order :

MyBioSource product webpage