ELISA/assay

Rat deoxypyridinoline (DPD) ELISA Kit

MBS263828

48-Strip-Wells

290 USD

ELISA Kit

Rat deoxypyridinoline (DPD) ELISA Kit

deoxypyridinoline (DPD)

Dihydropyrimidine dehydrogenase; Dihydropyrimidine dehydrogenase [NADP(+)]; dihydropyrimidine dehydrogenase [NADP(+)]; dihydropyrimidine dehydrogenase [NADP(+)]; dihydrouracil dehydrogenase; dihydrothymine dehydrogenase; dihydropyrimidine dehydrogenase; Dihydrothymine dehydrogenase; Dihydrouracil dehydrogenase

DPD

DPYD; DPYD; DHP; DPD; DHPDHASE; DHPDHase; DPD

DPYD_HUMAN

Rat

1025

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant DPD concentration. Assay Type: Sandwich. Detection Range: 500 nmol/L-7.8 nmol/L. Sensitivity: 1 nmol/L.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat DPD monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

160332325

Q12882.2

18,936 Da

Drug Metabolism - Other Enzymes Pathway (83033); Drug Metabolism - Other Enzymes Pathway (428); Fluoropyrimidine Activity Pathway (198778); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Pantothenate And CoA Biosynthesis Pathway (83015); Pantothenate And CoA Biosynthesis Pathway (401); Pyrimidine Catabolism Pathway (106288); Pyrimidine Degradation, Uracil = Beta-alanine, Thymine = 3-aminoisobutanoate Pathway (605866)

The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

DPYD: Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil. Homodimer. Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells. Belongs to the dihydropyrimidine dehydrogenase family. Protein type: Nucleotide Metabolism - pyrimidine; EC 1.3.1.2; Xenobiotic Metabolism - drug metabolism - other enzymes; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Other Amino Acids Metabolism - beta-alanine; Oxidoreductase. Chromosomal Location of Human Ortholog: 1p22. Cellular Component: cytoplasm; cytosol. Molecular Function: protein homodimerization activity; FAD binding; dihydroorotate dehydrogenase activity; metal ion binding; 4 iron, 4 sulfur cluster binding; dihydropyrimidine dehydrogenase (NADP+) activity; NADP binding. Biological Process: pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; uracil catabolic process; UMP biosynthetic process; beta-alanine biosynthetic process; thymine catabolic process; purine base catabolic process; thymidine catabolic process; pyrimidine base catabolic process. Disease: Dihydropyrimidine Dehydrogenase Deficiency

48-Strip-Wells

290 USD

96-Strip-Wells

450