catalog number :
MBS263717
products type :
ELISA Kit
products full name :
Human Pepsin ELISA Kit
products short name :
Pepsin
other names :
pepsin A-5 preproprotein; Pepsin A-5; pepsin A-5; pepsinogen-5; pepsinogen A5; pepsinogen 5, group I (pepsinogen A); Pepsinogen-5
other gene names :
PGA5; PGA5
uniprot entry name :
PEPA5_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PP concentration. Assay Type: Sandwich. Detection Range: 2000 pg/ml-31.2 pg/ml. Sensitivity: 12 pg/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human PP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_055039.1
ncbi gb acc num :
NM_014224.3
ncbi mol weight :
41,993 Da
ncbi pathways :
Protein Digestion And Absorption Pathway (172847); Protein Digestion And Absorption Pathway (171868)
ncbi summary :
This gene encodes a protein precursor of the digestive enzyme pepsin, a member of the peptidase A1 family of endopeptidases. The encoded precursor is secreted by gastric chief cells and undergoes autocatalytic cleavage in acidic conditions to form the active enzyme, which functions in the digestion of dietary proteins. This gene is found in a cluster of related genes on chromosome 11, each of which encodes one of multiple pepsinogens. Pepsinogen levels in serum may serve as a biomarker for atrophic gastritis and gastric cancer. [provided by RefSeq, Jul 2015]
uniprot summary :
PGA5: Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. Belongs to the peptidase A1 family. Protein type: Secreted; Secreted, signal peptide; EC 3.4.23.1. Chromosomal Location of Human Ortholog: 11q13. Molecular Function: aspartic-type endopeptidase activity. Biological Process: digestion; proteolysis