ELISA/assay

Rat inducible nitric oxide synthase (iNOS) ELISA Kit

MBS263618

48-Strip-Wells

290 USD

ELISA Kit

Rat inducible nitric oxide synthase (iNOS) ELISA Kit

inducible nitric oxide synthase (iNOS)

inducible nitric oxide synthase; Nitric oxide synthase, inducible; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2

iNOS

Nos2; Inducible NOS; iNOS

NOS2_RAT

Rat

1147

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant iNOS concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat iNOS monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

2935553

AAC83553.1

130,628 Da

iNOS: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. Homodimer. Binds SLC9A3R1. By endotoxins and cytokines. Induced by IFNG/IFN-gamma acting synergistically with bacterial lipopolysaccharides (LPS), TNF or IL1B/interleukin-1 beta. Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity. Belongs to the NOS family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 1.14.13.39; Amino Acid Metabolism - arginine and proline; Oxidoreductase. Cellular Component: cortical cytoskeleton; extracellular space; perinuclear region of cytoplasm; cytoplasm; peroxisome; vesicle membrane; intracellular; cytosol; nucleus. Molecular Function: protein homodimerization activity; FAD binding; nitric-oxide synthase activity; beta-catenin binding; cAMP-dependent protein kinase regulator activity; Hsp90 protein binding; actin binding; protein kinase binding; calmodulin binding; amino acid binding; NADPH-hemoprotein reductase activity; FMN binding; cadherin binding; iron ion binding; heme binding; nitric-oxide synthase binding; NADP binding; receptor binding. Biological Process: retinoic acid receptor signaling pathway; wound healing; positive regulation of apoptosis; cGMP-mediated signaling; response to hormone stimulus; response to lipopolysaccharide; positive regulation of killing of cells of another organism; positive regulation of vasodilation; signal transduction; defense response to Gram-negative bacterium; response to estradiol stimulus; regulation of heart contraction; regulation of blood pressure; negative regulation of blood pressure; nitric oxide biosynthetic process; inflammatory response; glycoside metabolic process; aging; superoxide metabolic process; positive regulation of guanylate cyclase activity; ovulation from ovarian follicle; regulation of cell proliferation; response to mechanical stimulus; endothelial cell proliferation; defense response to bacterium; response to hypoxia; peptidyl-cysteine S-nitrosylation; arginine catabolic process; blood vessel remodeling; G-protein signaling, coupled to cGMP nucleotide second messenger; response to activity; regulation of protein kinase activity; negative regulation of protein catabolic process; regulation of insulin secretion; nitric oxide mediated signal transduction

48-Strip-Wells

290 USD

96-Strip-Wells

450