catalog number :
MBS263466
products type :
ELISA Kit
products full name :
Rat Estradiol (E2) ELISA Kit
products short name :
Estradiol (E2)
other names :
estradiol 17-beta-dehydrogenase 1; Estradiol 17-beta-dehydrogenase 1; estradiol 17-beta-dehydrogenase 1; E2DH; 20-alpha-HSD; 17-beta-HSD 1; estradiol 17-beta-dehydrogenase-1; 20 alpha-hydroxysteroid dehydrogenase; 17-beta-hydroxysteroid dehydrogenase type 1; placental 17-beta-hydroxysteroid dehydrogenase; hydroxysteroid (17-beta) dehydrogenase 1 isoform; short chain dehydrogenase/reductase family 28CE, member 1; hydroxysteroid (17-beta) dehydrogenase 1; 17-beta-hydroxysteroid dehydrogenase type 1; 17-beta-HSD 1; 20 alpha-hydroxysteroid dehydrogenase; 20-alpha-HSD; E2DH; Placental 17-beta-hydroxysteroid dehydrogenase
other gene names :
HSD17B1; HSD17B1; HSD17; EDHB17; EDH17B2; SDR28C1; E17KSR; EDH17B1; EDH17B2; EDHB17; 17-beta-HSD 1; 20-alpha-HSD
uniprot entry name :
DHB1_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant E2 concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6 pg/ml. Sensitivity: 5 pg/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat E2 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_000404.2
ncbi gb acc num :
NM_000413.2
ncbi mol weight :
34,950 Da
ncbi pathways :
Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); Estrogen Biosynthesis Pathway (106155); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Metabolism Of Steroid Hormones And Vitamin D Pathway (106150); Ovarian Steroidogenesis Pathway (790011); Ovarian Steroidogenesis Pathway (795173); Prostate Cancer Pathway (755440)
uniprot summary :
HSD17B1: Favors the reduction of estrogens and androgens. Also has 20-alpha-HSD activity. Uses preferentially NADH. Belongs to the short-chain dehydrogenases/reductases (SDR) family. Protein type: EC 1.1.1.62; Oxidoreductase; Lipid Metabolism - androgen and estrogen. Chromosomal Location of Human Ortholog: 17q11-q21. Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; cytosol. Molecular Function: estradiol 17-beta-dehydrogenase activity; catalytic activity; 3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity. Biological Process: steroid metabolic process; estrogen metabolic process; estrogen biosynthetic process; steroid biosynthetic process