ELISA/assay

Human 15-lipoxygenase (15-LO / LOX) ELISA Kit

MBS263359

48-Strip-Wells

250 USD

ELISA Kit

Human 15-lipoxygenase (15-LO / LOX) ELISA Kit

[15-lipoxygenase (15-LO / LOX)]

[15-lipoxygenase; Arachidonate 15-lipoxygenase; arachidonate 15-lipoxygenase; 15-LOX; 12/15-lipoxygenase; 15-lipooxygenase-1; arachidonate omega-6 lipoxygenase; arachidonate 12-lipoxygenase, leukocyte-type; arachidonate 15-lipoxygenase; 12/15-lipoxygenase; Arachidonate 12-lipoxygenase, leukocyte-type (EC:1.13.11.31); 12-LOX; Arachidonate omega-6 lipoxygenase]

[15-LO / LOX]

[ALOX15; ALOX15; 12-LOX; 15LOX-1; 15-LOX-1; LOG15; 15-LOX; 15-LOX-1; 12-LOX]

LOX15_HUMAN

Human

662

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Human Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant 15-Epi-Lxa4 Concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human 15-epi-LXA4 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

307135

AAA36182.1

74,804 Da

Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (685553); Arachidonic Acid Metabolism Pathway (366); Eicosanoid Synthesis Pathway (198888); IL4-mediated Signaling Events Pathway (137933); Linoleic Acid Metabolism Pathway (82992); Linoleic Acid Metabolism Pathway (367); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Serotonergic Synapse Pathway (525336)

ALOX15: Converts arachidonic acid to 15S- hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid. Belongs to the lipoxygenase family. Protein type: Motility/polarity/chemotaxis; EC 1.13.11.33; Oxidoreductase; Lipid Metabolism - arachidonic acid; Lipid Metabolism - linoleic acid; EC 1.13.11.31. Chromosomal Location of Human Ortholog: 17p13.3. Cellular Component: extrinsic to internal side of plasma membrane; membrane; plasma membrane; lipid particle; cytosol. Molecular Function: phosphatidylinositol-4,5-bisphosphate binding; arachidonate 12-lipoxygenase activity; protein binding; hepoxilin A3 synthase activity; arachidonate 15-lipoxygenase activity; hepoxilin-epoxide hydrolase activity; iron ion binding. Biological Process: ossification; positive regulation of heterotypic cell-cell adhesion; wound healing; phosphatidylethanolamine biosynthetic process; leukotriene metabolic process; bone mineralization; positive regulation of actin filament polymerization; lipoxygenase pathway; negative regulation of adaptive immune response; arachidonic acid metabolic process; hepoxilin biosynthetic process; inflammatory response; apoptotic cell clearance

48-Strip-Wells

250 USD

96-Strip-Wells

365

5x96-Strip-Wells

1480

10x96-Strip-Wells

2620