ELISA/assay

Human Melanocyte antibody (MC Ab) ELISA Kit

MBS263351

48-Strip-Wells

275 USD

ELISA Kit

Human Melanocyte antibody (MC Ab) ELISA Kit

Melanocyte antibody (MC Ab)

Melanocyte protein PMEL; Melanocyte protein PMEL; melanocyte protein PMEL; melanocyte protein mel 17; silver, mouse, homolog of; melanocyte protein Pmel 17; melanosomal matrix protein17; silver locus protein homolog; melanoma-associated ME20 antigen; melanocytes lineage-specific antigen GP100; premelanosome protein; ME20-M; ME20M; Melanocyte protein Pmel 17; Melanocytes lineage-specific antigen GP100; Melanoma-associated ME20 antigen; P1; P100; Premelanosome protein; Silver locus protein homologCleaved into the following 2 chains:M-alphaAlternative name(s):95 kDa melanocyte-specific secreted glycoprotein; P26; Secreted melanoma-associated ME20 antigen; ME20-S; ME20S

MC Ab

PMEL; PMEL; P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E; D12S53E; PMEL17; SILV; ME20M; ME20-S; ME20S

PMEL_HUMAN

Human

661

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant MC Ab concentration. Assay Type: Sandwich. Detection Range: 1000 U/ml-15.6 U/ml

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human MC Ab monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

2507099

P40967.2

66,729 Da

This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011]

SILV: Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity. Belongs to the PMEL/NMB family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Vesicle. Chromosomal Location of Human Ortholog: 12q13-q14. Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; melanosome; extracellular region; plasma membrane; integral to membrane. Molecular Function: protein binding. Biological Process: melanin biosynthetic process; melanosome organization and biogenesis

48-Strip-Wells

275 USD

96-Strip-Wells

420