ELISA/assay

Mouse Melanocyte Stimulating Hormone (MSH) ELISA Kit

MBS263324

48-Strip-Wells

260 USD

ELISA Kit

Mouse Melanocyte Stimulating Hormone (MSH) ELISA Kit

[Melanocyte Stimulating Hormone (MSH)]

[Melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor); Melanocyte-stimulating hormone receptor; melanocyte-stimulating hormone receptor; MC1-R; melanotropin receptor; melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor); Melanocortin receptor 1; MC1-R]

[MSH]

[MC1R; MC1R; CMM5; MSH-R; SHEP2; MSHR; MSH-R; MC1-R]

MSHR_HUMAN

Mouse

317

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Mouse Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Mr-Proadm Concentration. Assay Type: Sandwich. Detection Range: 2000 pg/ml-31.2 pg/ml. Sensitivity: 12 pg/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse MR-ProADM monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

51593766

AAH80622.1

34,706 Da

Chaperonin-mediated Protein Folding Pathway (106242); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway (106243); Formation Of Tubulin Folding Intermediates By CCT/TriC Pathway (106245); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Melanogenesis Pathway (83092); Melanogenesis Pathway (504)

This intronless gene encodes the receptor protein for melanocyte-stimulating hormone (MSH). The encoded protein, a seven pass transmembrane G protein coupled receptor, controls melanogenesis. Two types of melanin exist: red pheomelanin and black eumelanin. Gene mutations that lead to a loss in function are associated with increased pheomelanin production, which leads to lighter skin and hair color. Eumelanin is photoprotective but pheomelanin may contribute to UV-induced skin damage by generating free radicals upon UV radiation. Binding of MSH to its receptor activates the receptor and stimulates eumelanin synthesis. This receptor is a major determining factor in sun sensitivity and is a genetic risk factor for melanoma and non-melanoma skin cancer. Over 30 variant alleles have been identified which correlate with skin and hair color, providing evidence that this gene is an important component in determining normal human pigment variation. [provided by RefSeq, Jul 2008]

MC1R: Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Genetic variations in MC1R are a cause of susceptibility to cutaneous malignant melanoma type 5 (CMM5). Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites. Belongs to the G-protein coupled receptor 1 family. Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR. Chromosomal Location of Human Ortholog: 16q24.3. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: protein binding; melanocortin receptor activity; hormone binding; melanocyte stimulating hormone receptor activity; ubiquitin protein ligase binding; peptide receptor activity, G-protein coupled. Biological Process: melanin biosynthetic process; positive regulation of protein kinase B signaling cascade; G-protein signaling, coupled to cyclic nucleotide second messenger; pigmentation; UV protection; multicellular organismal development; positive regulation of cAMP biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; sensory perception of pain. Disease: Albinism, Oculocutaneous, Type Ii; Increased Analgesia From Kappa-opioid Receptor Agonist, Female-specific; Melanoma, Cutaneous Malignant, Susceptibility To, 5; Skin/hair/eye Pigmentation, Variation In, 2

48-Strip-Wells

260 USD

96-Strip-Wells

405

5x96-Strip-Wells

1615

10x96-Strip-Wells

2880