catalog number :
MBS263232
products type :
ELISA Kit
products full name :
Human Mucin 5 Subtype AC (MUC5AC) ELISA Kit
products short name :
Mucin 5 Subtype AC (MUC5AC)
other names :
Mucin-5AC; Mucin-5AC; mucin-5AC; mucin 5AC, oligomeric mucus/gel-forming; gastric mucin; tracheobronchial mucin; major airway glycoprotein; lewis B blood group antigen; mucin-5 subtype AC, tracheobronchial; mucin 5AC, oligomeric mucus/gel-forming pseudogene; mucin 5, subtypes A and C, tracheobronchial/gastric; mucin 5AC, oligomeric mucus/gel-forming; Gastric mucin; Lewis B blood group antigen; LeB; Major airway glycoprotein; Mucin-5 subtype AC, tracheobronchial; Tracheobronchial mucin; TBM
products gene name :
MUC5AC
other gene names :
MUC5AC; MUC5AC; TBM; leB; MUC5; MUC5; MUC-5AC; LeB; TBM
uniprot entry name :
MUC5A_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant MUC5AC concentration. Assay Type: Sandwich. Detection Range: 5000 pg/ml-78 pg/ml. Sensitivity: 20 pg/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human MUC5AC monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi mol weight :
526,608 Da
ncbi pathways :
Metabolism Of Proteins Pathway (106230); O-linked Glycosylation Pathway (980459); O-linked Glycosylation Of Mucins Pathway (530747); Post-translational Protein Modification Pathway (161012); Termination Of O-glycan Biosynthesis Pathway (530748)
uniprot summary :
MUC5AC iso1: . Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: Golgi lumen; fibril. Molecular Function: extracellular matrix structural constituent. Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; fibril organization and biogenesis; post-translational protein modification