Human Cyclic Adenosine Monophosphate (cAMP) ELISA Kit | MyBioSource MBS263073 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Cyclic Adenosine Monophosphate (cAMP) ELISA Kit

catalog :

MBS263073

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS263073

products type :

ELISA Kit

products full name :

Human Cyclic Adenosine Monophosphate (cAMP) ELISA Kit

products short name :

Cyclic Adenosine Monophosphate (cAMP)

other names :

cyclic AMP-responsive element-binding protein 1 isoform B; Cyclic AMP-responsive element-binding protein 1; cyclic AMP-responsive element-binding protein 1; CREB-1; transactivator protein; active transcription factor CREB; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; cAMP responsive element binding protein 1

products gene name :

cAMP

other gene names :

CREB1; CREB1; CREB; CREB-1; cAMP-responsive element-binding protein 1

uniprot entry name :

CREB1_HUMAN

reactivity :

Human

sequence length :

341

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant cAMP concentration. Assay Type: Sandwich. Detection Range: 200 pmol/ml-3.12 pmol/ml. Sensitivity: 0.6 pmol/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Human cAMP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

19745184

ncbi acc num :

NP_604391.1

ncbi gb acc num :

NM_134442.3

ncbi mol weight :

25,445 Da

ncbi pathways :

AKT Phosphorylates Targets In The Nucleus Pathway (106476); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); ATF-2 Transcription Factor Network Pathway (138006); ATM Signaling Pathway (920969); Activated TLR4 Signalling Pathway (106400); Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (161033); Adaptive Immune System Pathway (366160); Adipogenesis Pathway (198832); Adrenergic Signaling In Cardiomyocytes Pathway (908257)

ncbi summary :

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

uniprot summary :

CREB: a transcription factor of the leucine zipper family of DNA binding proteins. Binds as a homodimer to the cAMP-responsive element (CRE). Phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Two splice-variant isoforms have been described. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 2q34. Cellular Component: nucleoplasm; nucleus. Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; enzyme binding; transcription cofactor activity; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; circadian rhythm; lactation; axon guidance; response to glucagon stimulus; nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of osteoclast differentiation; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; positive regulation of multicellular organism growth; toll-like receptor 3 signaling pathway; signal transduction; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; response to organic substance; synaptic transmission; toll-like receptor 4 signaling pathway; response to drug; epidermal growth factor receptor signaling pathway; mitochondrion organization and biogenesis; Notch signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; protein stabilization; secretory granule organization and biogenesis; MyD88-independent toll-like receptor signaling pathway; organelle organization and biogenesis; positive regulation of hormone secretion; positive regulation of lipid biosynthetic process; memory; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; phospholipase C activation; pituitary gland development; toll-like receptor signaling pathway; innate immune response; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; regulation of cell size. Disease: Histiocytoma, Angiomatoid Fibrous

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

420

more info or order :

MyBioSource product webpage