catalog number :
MBS263065
products type :
ELISA Kit
products full name :
Human Complement 5 a (C5a) ELISA Kit
products short name :
[Complement 5 a (C5a)]
other names :
[complement component C5, partial; Complement component C5; Complement component C5]
products gene name :
[C5a]
uniprot entry name :
Q804B3_NOTVI
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant C5a concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: Up to 0.05 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human C5a monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
AAO38044.1
ncbi mol weight :
49,195 Da
size4 :
10x96-Strip-Wells