catalog number :
MBS263057
products type :
ELISA Kit
products full name :
Mouse Leukotriene D4 (LTD4) ELISA Kit
products short name :
Leukotriene D4 (LTD4)
other names :
leukotriene D4 receptor; Cysteinyl leukotriene receptor 1; cysteinyl leukotriene receptor 1; LTD4 receptor; cysteinyl leukotriene D4 receptor; cysteinyl leukotriene 1 receptor short isoform; cysteinyl leukotriene receptor 1; Cysteinyl leukotriene D4 receptor; LTD4 receptor
products gene name :
LTD4
other gene names :
Cysltr1; Cysltr1; Cyslt1; CysLT1R; BB147369; Cyslt1; Cyslt1r; CysLTR1; LTD4 receptor
uniprot entry name :
CLTR1_MOUSE
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant LTD4 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse LTD4 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
AAF73047.1
ncbi mol weight :
39,160 Da
ncbi pathways :
Calcium Signaling Pathway (83247); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (1000942); Eicosanoid Ligand-binding Receptors Pathway (1000957); G Alpha (q) Signalling Events Pathway (1000975); GPCR Downstream Signaling Pathway (1000971); GPCR Ligand Binding Pathway (1000941); GPCRs, Class A Rhodopsin-like Pathway (198308); GPCRs, Other Pathway (198282); Gastrin-CREB Signalling Pathway Via PKC And MAPK (970815)
uniprot summary :
CYSLTR1: Receptor for cysteinyl leukotrienes mediating bronchoconstriction of individuals with and without asthma. Stimulation by LTD4 results in the contraction and proliferation of smooth muscle, edema, eosinophil migration and damage to the mucus layer in the lung. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTD4 LTE4 = LTC4 LTB4. Belongs to the G-protein coupled receptor 1 family. Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR. Cellular Component: membrane; integral to plasma membrane; integral to membrane; plasma membrane. Molecular Function: G-protein coupled receptor activity; signal transducer activity; leukotriene receptor activity. Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; cell surface receptor linked signal transduction; calcium ion transport; positive regulation of vasoconstriction; signal transduction; inflammatory response; chemotaxis