ELISA/assay

Human soluble vascular endothelial growth factor receptor 2 (sVEGFR-2) ELISA Kit

MBS262972

48-Strip-Wells

275 USD

ELISA Kit

Human soluble vascular endothelial growth factor receptor 2 (sVEGFR-2) ELISA Kit

soluble vascular endothelial growth factor receptor 2 (sVEGFR-2)

soluble vascular endothelial growth factor receptor 2; Vascular endothelial growth factor receptor 2; vascular endothelial growth factor receptor 2; kinase NYK; VEGF receptor-2; fetal liver kinase 1; protein-tyrosine kinase receptor flk-1; vascular endothelial growth factor receptor-2; vascular endothelial growth factor receptor-3; vascular endothelial growth factor receptor- 2; soluble vascular endothelial growth factor receptor 2; kinase insert domain protein receptor; Fetal liver kinase 1; FLK-1; Kinase NYK; Protein-tyrosine kinase receptor flk-1; CD_antigen: CD309

sVEGFR-2

Kdr; Kdr; Flk1; Ly73; Flk-1; Krd-1; VEGFR2; VEGFR-2; sVEGFR-2; 6130401C07; Flk-1; Flk1; VEGFR-2; FLK-1

VGFR2_MOUSE

Human

673

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant sVEGFR-2 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312ng/ml. Sensitivity: 0.6ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human sVEGFR-2 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

215400616

ACJ66293.1

75,230 Da

Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Endocytosis Pathway (102286); Endocytosis Pathway (102181); Focal Adhesion Pathway (83264); Focal Adhesion Pathway (478); Id Signaling Pathway (198411); Neurophilin Interactions With VEGF And VEGFR Pathway (819803); PI3K-Akt Signaling Pathway (692242); PI3K-Akt Signaling Pathway (692979)

VEGFR2: a receptor tyrosine kinase of the VEGFR family. High affinity receptor for VEGF and VEGF-C. Ligand binding induces autophosphorylation and activation. Activated receptor recruits proteins including Shc, GRB2, PI3K, Nck, SHP-1 and SHP-2. Plays a key role in vascular development and regulation of vascular permeability. Protein type: Protein kinase, TK; Kinase, protein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Membrane protein, integral; TK group; VEGFR family. Cellular Component: Golgi apparatus; neuron projection; cell surface; integral to plasma membrane; endoplasmic reticulum; early endosome; integral to membrane; extracellular region; cytosol; lipid raft; membrane; cell soma; cytoplasm; plasma membrane; cytoplasmic vesicle; nucleus; cell junction; endosome; external side of plasma membrane. Molecular Function: transferase activity; integrin binding; vascular endothelial growth factor receptor activity; protein binding; growth factor binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; protein kinase activity. Biological Process: positive regulation of positive chemotaxis; multicellular organismal development; cell maturation; positive regulation of vasodilation; protein amino acid phosphorylation; positive regulation of long-term neuronal synaptic plasticity; calcium ion homeostasis; regulation of cell shape; elevation of cytosolic calcium ion concentration; positive regulation of mesenchymal cell proliferation; negative regulation of neuron apoptosis; positive regulation of TOR signaling pathway; response to drug; regulation of endothelial cell proliferation; cell fate commitment; embryonic hemopoiesis; regulation of endothelial cell differentiation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of angiogenesis; branching morphogenesis of a tube; cell migration during sprouting angiogenesis; positive regulation of endothelial cell proliferation; lymph vessel development; alveolus development; surfactant homeostasis; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; positive regulation of nitric-oxide synthase biosynthetic process; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; positive regulation of calcium-mediated signaling; positive regulation of vascular endothelial growth factor receptor signaling pathway; ovarian follicle development; positive regulation of MAPKKK cascade; positive regulation of focal adhesion formation; positive regulation of cell proliferation; hemopoiesis; angiogenesis; vasculogenesis; cell differentiation; positive regulation of BMP signaling pathway; endothelial cell differentiation; cell migration; male gonad development; response to hypoxia; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; phosphorylation; neurite morphogenesis; lung development; positive regulation of cell migration

48-Strip-Wells

275 USD

96-Strip-Wells

420