ELISA/assay

Mouse angiostatin (ANG) ELISA Kit

MBS262880

48-Strip-Wells

290 USD

ELISA Kit

Mouse angiostatin (ANG) ELISA Kit

angiostatin (ANG)

angiomotin isoform 1; Angiomotin; angiomotin; angiomotin p80 isoform; angiomotin p130 isoform; angiomotin

ANG

AMOT; AMOT; KIAA1071

AMOT_HUMAN

Mouse

1084

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Mouse serum, plasma or cell culture supernatant and organizations in the natural and recombinant ANG concentration. Assay Type: Quantitative Sandwich. Sensitivity: Up to 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse ANG monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

166064029

NP_001106962.1

NM_001113490.1

72,540 Da

Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388); Signal Transduction Pathway (477114); Signaling By Hippo Pathway (576275)

This gene belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

AMOT: Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions. Belongs to the angiomotin family. 2 isoforms of the human protein are produced by alternative splicing. Chromosomal Location of Human Ortholog: Xq23. Cellular Component: signalosome; ruffle; cell surface; tight junction; lamellipodium; endocytic vesicle; cytoplasm; stress fiber; integral to membrane; actin filament; cytosol; external side of plasma membrane. Molecular Function: angiostatin binding; protein binding; receptor activity. Biological Process: intercellular junction assembly; blood vessel endothelial cell migration; positive regulation of cell size; in utero embryonic development; positive regulation of blood vessel endothelial cell migration; gastrulation with mouth forming second; chemotaxis; regulation of cell migration; negative regulation of angiogenesis; regulation of small GTPase mediated signal transduction; positive regulation of embryonic development; positive regulation of stress fiber formation; negative regulation of vascular permeability; actin cytoskeleton organization and biogenesis; vasculogenesis; cell migration involved in gastrulation

48-Strip-Wells

290 USD

96-Strip-Wells

450