ELISA/assay

Human Soluble Cluster of differentiation 40 ligand (sCD40L) ELISA KIT

MBS262870

48-Strip-Wells

275 USD

ELISA Kit

Human Soluble Cluster of differentiation 40 ligand (sCD40L) ELISA KIT

Soluble Cluster of differentiation 40 ligand (sCD40L)

Sodium/hydrogen exchanger 10; Sodium/hydrogen exchanger 10; sodium/hydrogen exchanger 10; sNHE; NHE-10; Na(+)/H(+) exchanger 10; solute carrier family 9 member C1; solute carrier family 10 member 10; solute carrier family 9, member 10; solute carrier family 9, isoform 10; sperm-specific Na(+)/H(+) exchanger; sperm-specific sodium proton exchanger; solute carrier family 9, subfamily C (Na+-transporting carboxylic acid decarboxylase), member 1; Na(+)/H(+) exchanger 10; NHE-10; Solute carrier family 9 member 10; Solute carrier family 9 member C1; Sperm-specific Na(+)/H(+) exchanger; sNHE

sCD40L

SLC9C1; SLC9C1; NHE; SLC9A10; sperm-NHE; SLC9A10; NHE-10; sNHE

SL9C1_HUMAN

Human

1177

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant sCD40L concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human sCD40L monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

158563886

Q4G0N8.2

129,365 Da

Ion Channel Transport Pathway (187193); Stimuli-sensing Channels Pathway (771568); Transmembrane Transport Of Small Molecules Pathway (106572)

SLC9A10 is a member of the sodium-hydrogen exchanger (NHE) family (see SLC9A1, MIM 107310) and is required for male fertility and sperm motility (Wang et al., 2003 [PubMed 14634667]).[supplied by OMIM, Apr 2009]

NHE10: Sperm-specific sodium/hydrogen exchanger involved in intracellular pH regulation of spermatozoa. Required for sperm motility and fertility. Involved in sperm cell hyperactivation, a step needed for sperm motility which is essential late in the preparation of sperm for fertilization. Required for the expression and bicarbonate regulation of the soluble adenylyl cyclase (sAC). Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter, ion channel. Chromosomal Location of Human Ortholog: 3q13.2. Cellular Component: integral to membrane; plasma membrane. Molecular Function: solute:hydrogen antiporter activity. Biological Process: sperm motility; multicellular organismal development; sodium ion transport; spermatogenesis; cell differentiation; transmembrane transport

48-Strip-Wells

275 USD

96-Strip-Wells

420