ELISA/assay

Mouse Hepatic lipase (HL) ELISA Kit

MBS262817

48-Strip-Wells

290 USD

ELISA Kit

Mouse Hepatic lipase (HL) ELISA Kit

Hepatic lipase (HL)

hepatic triacylglycerol lipase; Hepatic triacylglycerol lipase; hepatic triacylglycerol lipase; hepatic lipase; lipase member C; lipase, hepatic; Lipase member C

HL

LIPC; LIPC; HL; HTGL; LIPH; HDLCQ12; HTGL; HL; Hepatic lipase

LIPC_HUMAN

Mouse

499

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant HL concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse HL monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

194097335

NP_000227.2

NM_000236.2

55,914 Da

Acylglycerol Degradation Pathway (413388); Acylglycerol Degradation Pathway (468272); Chylomicron-mediated Lipid Transport Pathway (106157); Fatty Acid Beta Oxidation Pathway (198865); Glycerolipid Metabolism Pathway (82986); Glycerolipid Metabolism Pathway (361); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156); Metabolic Pathways (132956); Metabolism Pathway (477135)

LIPC encodes hepatic triglyceride lipase, which is expressed in liver. LIPC has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. [provided by RefSeq, Jul 2008]

LIPC: Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin. Defects in LIPC are the cause of hepatic lipase deficiency (HL deficiency). A disorder characterized by elevated levels of beta-migrating very low density lipoproteins, and abnormally triglyceride-rich low and high density lipoproteins. Belongs to the AB hydrolase superfamily. Lipase family. Protein type: Phospholipase; Secreted, signal peptide; Secreted; Lipid Metabolism - glycerolipid; EC 3.1.1.3. Chromosomal Location of Human Ortholog: 15q21-q23. Cellular Component: extracellular space. Molecular Function: heparin binding; triacylglycerol lipase activity; low-density lipoprotein binding; apolipoprotein binding; phospholipase activity. Biological Process: cholesterol metabolic process; cholesterol homeostasis; reverse cholesterol transport; triacylglycerol catabolic process; fatty acid biosynthetic process. Disease: High Density Lipoprotein Cholesterol Level Quantitative Trait Locus 12; Hepatic Lipase Deficiency; Diabetes Mellitus, Noninsulin-dependent

48-Strip-Wells

290 USD

96-Strip-Wells

450