Human inducible nitric oxide synthase (iNOS) ELISA Kit | MyBioSource MBS262750 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human inducible nitric oxide synthase (iNOS) ELISA Kit

catalog :

MBS262750

quantity :

48-Strip-Wells

price :

250 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS262750

products type :

ELISA Kit

products full name :

Human inducible nitric oxide synthase (iNOS) ELISA Kit

products short name :

[inducible nitric oxide synthase (iNOS)]

other names :

[inducible nitric oxide synthase; Nitric oxide synthase, inducible; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2]

products gene name :

[iNOS]

other gene names :

[Nos2; Inducible NOS; iNOS]

uniprot entry name :

NOS2_RAT

reactivity :

Human

sequence length :

1147

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Human Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Inh-B Concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6 pg/ml. Sensitivity: 5 pg/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human INH-B monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

2935553

ncbi acc num :

AAC83553.1

ncbi mol weight :

130,628 Da

uniprot summary :

iNOS: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. Homodimer. Binds SLC9A3R1. By endotoxins and cytokines. Induced by IFNG/IFN-gamma acting synergistically with bacterial lipopolysaccharides (LPS), TNF or IL1B/interleukin-1 beta. Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity. Belongs to the NOS family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 1.14.13.39; Amino Acid Metabolism - arginine and proline; Oxidoreductase. Cellular Component: cortical cytoskeleton; extracellular space; perinuclear region of cytoplasm; cytoplasm; peroxisome; vesicle membrane; intracellular; nucleus; cytosol. Molecular Function: protein homodimerization activity; FAD binding; nitric-oxide synthase activity; beta-catenin binding; cAMP-dependent protein kinase regulator activity; Hsp90 protein binding; actin binding; protein kinase binding; calmodulin binding; amino acid binding; NADPH-hemoprotein reductase activity; FMN binding; cadherin binding; iron ion binding; heme binding; nitric-oxide synthase binding; NADP binding; receptor binding. Biological Process: retinoic acid receptor signaling pathway; wound healing; cGMP-mediated signaling; positive regulation of apoptosis; response to hormone stimulus; response to lipopolysaccharide; positive regulation of killing of cells of another organism; defense response to Gram-negative bacterium; signal transduction; positive regulation of vasodilation; regulation of heart contraction; response to estradiol stimulus; regulation of blood pressure; negative regulation of blood pressure; inflammatory response; nitric oxide biosynthetic process; glycoside metabolic process; aging; superoxide metabolic process; positive regulation of guanylate cyclase activity; ovulation from ovarian follicle; regulation of cell proliferation; response to mechanical stimulus; endothelial cell proliferation; defense response to bacterium; response to hypoxia; peptidyl-cysteine S-nitrosylation; arginine catabolic process; G-protein signaling, coupled to cGMP nucleotide second messenger; blood vessel remodeling; response to activity; negative regulation of protein catabolic process; regulation of protein kinase activity; nitric oxide mediated signal transduction; regulation of insulin secretion

size1 :

48-Strip-Wells

price1 :

250 USD

size2 :

96-Strip-Wells

price2 :

365

size3 :

5x96-Strip-Wells

price3 :

1480

size4 :

10x96-Strip-Wells

price4 :

2620

more info or order :

MyBioSource product webpage