Human Breast Cancer Susceptibility Protein 2 (BRCA2) ELISA Kit | MyBioSource MBS262713 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Breast Cancer Susceptibility Protein 2 (BRCA2) ELISA Kit

catalog :

MBS262713

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS262713

products type :

ELISA Kit

products full name :

Human Breast Cancer Susceptibility Protein 2 (BRCA2) ELISA Kit

products short name :

Breast Cancer Susceptibility Protein 2 (BRCA2)

other names :

breast cancer type 2 susceptibility protein; Breast cancer type 2 susceptibility protein; breast cancer type 2 susceptibility protein; Fanconi anemia group D1 protein; breast cancer 2 tumor suppressor; BRCA1/BRCA2-containing complex, subunit 2; breast and ovarian cancer susceptibility gene, early onset; breast cancer 2, early onset; Fanconi anemia group D1 protein

products gene name :

BRCA2

other gene names :

BRCA2; BRCA2; FAD; FACD; FAD1; GLM3; BRCC2; FANCD; PNCA2; FANCD1; BROVCA2; FACD; FANCD1

uniprot entry name :

BRCA2_HUMAN

reactivity :

Human

sequence length :

3418

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant BRCA2 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human BRCA2 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

119395734

ncbi acc num :

NP_000050.2

ncbi gb acc num :

NM_000059.3

ncbi mol weight :

384,225 Da

ncbi pathways :

DNA Repair Pathway (105837); Double-Strand Break Repair Pathway (105861); FOXM1 Transcription Factor Network Pathway (137935); Fanconi Anemia Pathway (105895); Fanconi Anemia Pathway (377262); Fanconi Anemia Pathway (377128); Homologous DNA Pairing And Strand Exchange Pathway (105870); Homologous Recombination Repair Pathway (105862); Homologous Recombination Pathway (198890); Homologous Recombination Pathway (83046)

ncbi summary :

Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele. [provided by RefSeq, Dec 2008]

uniprot summary :

BRCA2: involved in double-strand break repair and/or homologous recombination. May participate in S phase checkpoint activation. Interacts with RAD51 and DSS1. Interacts with ubiquitinated FANCD2. Interacts with PALB2, enables the recombinational repair and checkpoint functions. Interacts with WDR16. Defects in BRCA2 are a cause of genetic susceptibility to breast cancer and may underlie susceptibility to uveal melanoma. Protein type: Tumor suppressor; Nuclear receptor co-regulator; DNA repair, damage. Chromosomal Location of Human Ortholog: 13q12.3. Cellular Component: nucleoplasm; centrosome; protein complex; cytoplasm; BRCA2-MAGE-D1 complex; nucleus; secretory granule. Molecular Function: H4 histone acetyltransferase activity; gamma-tubulin binding; protein binding; histone acetyltransferase activity; protease binding; H3 histone acetyltransferase activity; single-stranded DNA binding. Biological Process: positive regulation of transcription, DNA-dependent; cytokinesis; cell aging; DNA repair; positive regulation of mitotic cell cycle; regulation of cytokinesis; oocyte maturation; inner cell mass cell proliferation; double-strand break repair via homologous recombination; negative regulation of mammary gland epithelial cell proliferation; response to UV-C; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; male meiosis I; nucleotide-excision repair; double-strand break repair; response to gamma radiation; hemopoiesis; spermatogenesis; replication fork protection; brain development; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; centrosome duplication; female gonad development; response to X-ray. Disease: Pancreatic Cancer, Susceptibility To, 2; Breast-ovarian Cancer, Familial, Susceptibility To, 2; Prostate Cancer; Breast Cancer; Medulloblastoma; Glioma Susceptibility 3; Tracheoesophageal Fistula With Or Without Esophageal Atresia; Fanconi Anemia, Complementation Group D1; Wilms Tumor 1

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

420

more info or order :

MyBioSource product webpage