catalog number :
MBS262534
products type :
ELISA Kit
products full name :
Mouse Pancreatic Lipase (PL) ELISA Kit
products short name :
Pancreatic Lipase (PL)
other names :
pancreatic lipase; Pancreatic triacylglycerol lipase; pancreatic triacylglycerol lipase; triacylglycerol acylhydrolase; pancreatic lipase
other gene names :
PNLIP; PNLIP; PL; PTL; PNLIPD; PL; PTL; Pancreatic lipase
uniprot entry name :
LIPP_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PL concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6 pg/ml. Sensitivity: 5 pg/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse PL monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
AAA99053.1
ncbi mol weight :
51,157 Da
ncbi pathways :
Acylglycerol Degradation Pathway (413388); Acylglycerol Degradation Pathway (468272); Digestion Of Dietary Lipid Pathway (106112); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); Fat Digestion And Absorption Pathway (194385); Fat Digestion And Absorption Pathway (194324); Glycerolipid Metabolism Pathway (82986); Glycerolipid Metabolism Pathway (361); Lipid Digestion, Mobilization, And Transport Pathway (106111)
ncbi summary :
This gene is a member of the lipase gene family. It encodes a carboxyl esterase that hydrolyzes insoluble, emulsified triglycerides, and is essential for the efficient digestion of dietary fats. This gene is expressed specifically in the pancreas. [provided by RefSeq, Jul 2008]
uniprot summary :
PNLIP: a member of the lipase gene family. It encodes a carboxyl esterase that hydrolyzes insoluble, emulsified triglycerides, and is essential for the efficient digestion of dietary fats. expressed specifically in the pancreas. [provided by RefSeq, Jul 2008]. Protein type: Secreted; Lipid Metabolism - glycerolipid; EC 3.1.1.3; Hydrolase; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 10q26.1. Cellular Component: extracellular region. Molecular Function: triacylglycerol lipase activity; metal ion binding. Biological Process: phototransduction, visible light; cholesterol absorption; retinoid metabolic process; lipid catabolic process; lipid digestion. Disease: Pancreatic Lipase Deficiency