ELISA/assay

Human Thymidine Phosphorylase (TP) ELISA Kit

MBS262471

48-Strip-Wells

275 USD

ELISA Kit

Human Thymidine Phosphorylase (TP) ELISA Kit

Thymidine Phosphorylase (TP)

thymidine phosphorylase isoform 1 proprotein; Thymidine phosphorylase; thymidine phosphorylase; tdRPase; gliostatin; thymidine phosphorylase; Gliostatin; Platelet-derived endothelial cell growth factor; PD-ECGF; TdRPase

TP

TYMP; TYMP; TP; ECGF; ECGF1; MNGIE; MEDPS1; MTDPS1; PDECGF; hPD-ECGF; ECGF1; TP; PD-ECGF

TYPH_HUMAN

Human

482

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant TP concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: ? 8%. Inter-assay Precision: ? 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human TP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

384229049

NP_001244917.1

NM_001257988.1

50,405 Da

Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Drug Metabolism - Other Enzymes Pathway (83033); Drug Metabolism - Other Enzymes Pathway (428); Fluoropyrimidine Activity Pathway (198778); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Pyrimidine Catabolism Pathway (106288); Pyrimidine Metabolism Pathway (106281); Pyrimidine Metabolism Pathway (82946)

This gene encodes an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in this gene have been associated with mitochondrial neurogastrointestinal encephalomyopathy. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Apr 2012]

ECGF1: May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro. Homodimer. Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. Protein type: Cytokine; Transferase; Xenobiotic Metabolism - drug metabolism - other enzymes; Phosphorylase; EC 2.4.2.4; DNA replication; Nucleotide Metabolism - pyrimidine; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 22q13.33. Cellular Component: cytosol. Molecular Function: thymidine phosphorylase activity; growth factor activity; phosphorylase activity; transferase activity, transferring pentosyl groups; pyrimidine-nucleoside phosphorylase activity; platelet-derived growth factor receptor binding. Biological Process: pyrimidine nucleoside catabolic process; mitochondrial genome maintenance; pyrimidine base metabolic process; pyrimidine nucleotide metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside salvage; angiogenesis; cell differentiation; DNA replication; chemotaxis. Disease: Mitochondrial Dna Depletion Syndrome 1 (mngie Type)

48-Strip-Wells

275 USD

96-Strip-Wells

420