ELISA/assay

Human Serum Amyloid P Component (SAP) ELISA Kit

MBS262436

48-Strip-Wells

275 USD

ELISA Kit

Human Serum Amyloid P Component (SAP) ELISA Kit

Serum Amyloid P Component (SAP)

serum amyloid P-component; Serum amyloid P-component; serum amyloid P-component; pentaxin-related; 9.5S alpha-1-glycoprotein; epididymis secretory sperm binding protein Li 92n; amyloid P component, serum; 9.5S alpha-1-glycoproteinCleaved into the following chain:Serum amyloid P-component(1-203)

SAP

APCS; APCS; SAP; PTX2; HEL-S-92n; PTX2; SAP

SAMP_HUMAN

Human

223

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant SAP concentration. Assay Type: Sandwich. Detection Range: 500 ng/ml-7.8 ng/ml. Sensitivity: 1 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human SAP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

4502133

NP_001630.1

NM_001639.3

25,387 Da

Amyloids Pathway (366238); Disease Pathway (530764)

The protein encoded by this gene is a glycoprotein, belonging to the pentraxin family of proteins, which has a characteristic pentameric organization. These family members have considerable sequence homology which is thought to be the result of gene duplication. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo. [provided by RefSeq, Sep 2008]

APCS: Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin. SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits. Belongs to the pentaxin family. Protein type: Secreted, signal peptide; Secreted; Chaperone. Chromosomal Location of Human Ortholog: 1q21-q23. Cellular Component: extracellular matrix; extracellular space; protein complex; extracellular region; nucleus. Molecular Function: complement component C1q binding; unfolded protein binding; virion binding; calcium ion binding; carbohydrate binding. Biological Process: protein folding; negative regulation of virion penetration into host cell; negative regulation of acute inflammatory response; acute-phase response; innate immune response; negative regulation of monocyte differentiation; chaperone-mediated protein complex assembly; negative regulation of viral reproduction

48-Strip-Wells

275 USD

96-Strip-Wells

420