ELISA/assay

Chicken Aryl Hydrocarbon Receptor (AhR) ELISA Kit

MBS262394

48-Strip-Wells

315 USD

ELISA Kit

Chicken Aryl Hydrocarbon Receptor (AhR) ELISA Kit

Aryl Hydrocarbon Receptor (AhR)

aryl hydrocarbon receptor; Aryl hydrocarbon receptor; aryl hydrocarbon receptor; AH-receptor; ah receptor; aromatic hydrocarbon receptor; class E basic helix-loop-helix protein 76; aryl hydrocarbon receptor; Class E basic helix-loop-helix protein 76; bHLHe76

AhR

AHR; AHR; bHLHe76; BHLHE76; Ah receptor; AhR; bHLHe76

AHR_HUMAN

Chicken

848

Store all reagents at 2-8 degree C.

Samples: Chicken serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant AhR concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Chicken AhR monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

4502003

NP_001612.1

NM_001621.4

96,147 Da

Adipogenesis Pathway (198832); AhR Pathway (755436); Integrated Breast Cancer Pathway (219801)

This gene encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Its ligands included a variety of aromatic hydrocarbons. [provided by RefSeq, Jul 2008]

AHR: a nuclear receptor for aryl hydrocarbons involved in xenobiotic metabolism, cell cycle regulation, and development in response to both endogenous and environmental signals. AhR was initially identified as a receptor for dioxins, which are environmental pollutants generated by waste incineration and other industrial processes . AhR ligands include polycyclic aromatic hydrocarbons, including the carcinogen benzo(a)pyrene and other components of cigarette smoke. Naturally occurring AhR ligands include flavonoids, which are aromatic plant secondary compounds commonly found in vegetables and fruits. Cytoplasmic aryl hydrocarbon receptors are found in protein complexes with heat shock proteins. Upon ligand binding, AhR dissociates from heat shock proteins and translocate to the nucleus where it dimerizes with AhR nuclear translocator (ARNT, HIF-1b). The AhR/ARNT heterodimer binds to nuclear xenobiotic response elements to control the expression of genes associated with xenobiotic metabolism, including several cytochrome P450 genes. AhR is ubiquitously expressed and is thought to play a role in regulation of cell proliferation and differentiation, cytokine expression, and xenobiotic metabolism. Research studies link AhR activity with the control of regulatory T-cell and T-helper 17 cell differentiation, regulation of the inflammatory response, and the onset of lung cancer. Protein type: Nuclear receptor; Transcription factor; DNA-binding. Chromosomal Location of Human Ortholog: 7p15. Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus. Molecular Function: protein dimerization activity; RNA polymerase II transcription factor activity, enhancer binding; ligand-dependent nuclear receptor activity; signal transducer activity; protein binding; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; Hsp90 protein binding; transcription factor binding; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; prostate gland development; blood vessel development; intracellular receptor-mediated signaling pathway; apoptosis; positive regulation of transcription, DNA-dependent; response to toxin; negative regulation of transcription from RNA polymerase II promoter; cell cycle; regulation of transcription from RNA polymerase II promoter; response to xenobiotic stimulus; regulation of transcription, DNA-dependent; regulation of gene expression; xenobiotic metabolic process; positive regulation of transcription from RNA polymerase II promoter; regulation of B cell proliferation; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent; positive regulation of transcriptional preinitiation complex assembly

48-Strip-Wells

315 USD

96-Strip-Wells

490