Rat Phospholipase A2 (PLA2) ELISA Kit | MyBioSource MBS262388 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Phospholipase A2 (PLA2) ELISA Kit

catalog :

MBS262388

quantity :

48-Strip-Wells

price :

290 USD

more info or order :

MyBioSource product webpage

citations: 1

Reference
Eftekhar N, Moghimi A, Mohammadian Roshan N, Saadat S, Boskabady M. Immunomodulatory and anti-inflammatory effects of hydro-ethanolic extract of Ocimum basilicum leaves and its effect on lung pathological changes in an ovalbumin-induced rat model of asthma. BMC Complement Altern Med. 2019;19:349 pubmed publisher

product information

catalog number :

MBS262388

products type :

ELISA Kit

products full name :

Rat Phospholipase A2 (PLA2) ELISA Kit

products short name :

Phospholipase A2 (PLA2)

other names :

phospholipase A2; Phospholipase A2; phospholipase A2; phosphatidylcholine 2-acylhydrolase 1B; phospholipase A2, group IB (pancreas); Group IB phospholipase A2; Phosphatidylcholine 2-acylhydrolase 1B

products gene name :

PLA2

other gene names :

PLA2G1B; PLA2G1B; PLA2; PLA2A; PPLA2; PLA2; PLA2A; PPLA2

uniprot entry name :

PA21B_HUMAN

reactivity :

Rat

sequence length :

148

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Rat serum, plasma or cell culture supernatant and organizations in the natural and recombinant PLA2 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: Up to 0.06 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat PLA2 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

4505847

ncbi acc num :

NP_000919.1

ncbi gb acc num :

NM_000928.2

ncbi mol weight :

16,360 Da

ncbi pathways :

Acyl Chain Remodelling Of PC Pathway (645321); Acyl Chain Remodelling Of PE Pathway (645317); Acyl Chain Remodelling Of PG Pathway (645328); Acyl Chain Remodelling Of PI Pathway (645326); Acyl Chain Remodelling Of PS Pathway (645324); Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (366); Ether Lipid Metabolism Pathway (82990); Ether Lipid Metabolism Pathway (365); Fat Digestion And Absorption Pathway (194385)

ncbi summary :

This gene encodes a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. The encoded calcium-dependent enzyme catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids. AA is subsequently converted by downstream metabolic enzymes to several bioactive lipophilic compounds (eicosanoids), including prostaglandins (PGs) and leukotrienes (LTs). The enzyme may be involved in several physiological processes including cell contraction, cell proliferation and pathological response. [provided by RefSeq, Aug 2013]

uniprot summary :

PLA2G1B: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Belongs to the phospholipase A2 family. Protein type: Secreted; Secreted, signal peptide; Lipid Metabolism - ether lipid; Phospholipase; DNA replication; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - linoleic acid; Lipid Metabolism - arachidonic acid; Lipid Metabolism - alpha-linolenic acid; EC 3.1.1.4. Chromosomal Location of Human Ortholog: 12q24.31. Cellular Component: extracellular space; cell surface; extracellular region; secretory granule. Molecular Function: phospholipase A2 activity; calcium-dependent phospholipase A2 activity; bile acid binding; calcium ion binding; receptor binding. Biological Process: positive regulation of immune response; activation of MAPK activity; neutrophil mediated immunity; activation of phospholipase A2; glucose transport; signal transduction; phosphatidylcholine metabolic process; activation of NF-kappaB transcription factor; positive regulation of fibroblast proliferation; phospholipid metabolic process; fatty acid biosynthetic process; multicellular organismal lipid catabolic process; neutrophil chemotaxis; glycerophospholipid biosynthetic process; actin filament organization; arachidonic acid secretion; leukotriene biosynthetic process; defense response to Gram-positive bacterium; innate immune response in mucosa; positive regulation of protein secretion; cellular response to insulin stimulus; antibacterial humoral response; phosphatidic acid biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; interleukin-8 production; positive regulation of DNA replication

size1 :

48-Strip-Wells

price1 :

290 USD

size2 :

96-Strip-Wells

price2 :

450

more info or order :

MyBioSource product webpage