Rat alpha2-Antiplasmin (alpha2-AP) ELISA Kit | MyBioSource MBS262375 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat alpha2-Antiplasmin (alpha2-AP) ELISA Kit

catalog :

MBS262375

quantity :

48-Strip-Wells

price :

290 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS262375

products type :

ELISA Kit

products full name :

Rat alpha2-Antiplasmin (alpha2-AP) ELISA Kit

products short name :

alpha2-Antiplasmin (alpha2-AP)

products name syn :

Rat a2-Antiplasmin (a2-AP) ELISA Kit

other names :

alpha-2-antiplasmin isoform a; Alpha-2-antiplasmin; alpha-2-antiplasmin; serpin F2; alpha-2-AP; alpha-2-plasmin inhibitor; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2; Alpha-2-plasmin inhibitor; Alpha-2-PI; Serpin F2

products gene name :

alpha2-AP

other gene names :

SERPINF2; SERPINF2; AAP; API; PLI; A2AP; ALPHA-2-PI; AAP; PLI; Alpha-2-AP; Alpha-2-PI

uniprot entry name :

A2AP_HUMAN

reactivity :

Rat

sequence length :

491

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Rat serum, plasma or cell culture supernatant and organizations in the natural and recombinant alpha2-AP concentration. Assay Type: Quantitative Sandwich. Sensitivity: Up to 0.06 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat alpha2-AP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

115583663

ncbi acc num :

NP_000925.2

ncbi gb acc num :

NM_000934.3

ncbi mol weight :

47,907 Da

ncbi pathways :

Blood Clotting Cascade Pathway (198840); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Dissolution Of Fibrin Clot Pathway (106061); Hemostasis Pathway (106028); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048)

ncbi summary :

This gene encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

uniprot summary :

SERPINF2: Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin. Defects in SERPINF2 are the cause of alpha-2-plasmin inhibitor deficiency (APLID). APLID is an autosomal recessive disorder resulting in severe hemorrhagic diathesis. Belongs to the serpin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 17p13. Cellular Component: extracellular space; cell surface; fibrinogen complex; extracellular region. Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protein homodimerization activity; protease binding; endopeptidase inhibitor activity. Biological Process: platelet activation; collagen fibril organization; positive regulation of smooth muscle cell proliferation; positive regulation of collagen biosynthetic process; positive regulation of JNK cascade; response to organic substance; fibrinolysis; negative regulation of fibrinolysis; platelet degranulation; renin-angiotensin regulation of blood vessel size; positive regulation of stress fiber formation; acute-phase response; blood vessel morphogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation; blood coagulation. Disease: Alpha-2-plasmin Inhibitor Deficiency

size1 :

48-Strip-Wells

price1 :

290 USD

size2 :

96-Strip-Wells

price2 :

450

more info or order :

MyBioSource product webpage