ELISA/assay

Rat Oxidized Low Density Lipoprotein (OxLDL) ELISA Kit

MBS262297

48-Strip-Wells

260 USD

ELISA Kit

Rat Oxidized Low Density Lipoprotein (OxLDL) ELISA Kit

[Oxidized Low Density Lipoprotein (OxLDL)]

[Oxidized low density lipoprotein (lectin-like) receptor 1; Oxidized low-density lipoprotein receptor 1; oxidized low-density lipoprotein receptor 1; hLOX-1; ox LDL receptor 1; lectin-type oxidized LDL receptor 1; scavenger receptor class E, member 1; C-type lectin domain family 8 member A; oxidized low-density lipoprotein receptor 1, soluble form; oxidized low density lipoprotein (lectin-like) receptor 1; C-type lectin domain family 8 member A; Lectin-like oxidized LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; hLOX-1; Lectin-type oxidized LDL receptor 1Cleaved into the following chain:Oxidized low-density lipoprotein receptor 1, soluble form]

[OxLDL]

[OLR1; OLR1; LOX1; LOXIN; SLOX1; CLEC8A; SCARE1; CLEC8A; LOX1; Ox-LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; hLOX-1]

OLR1_HUMAN

Rat

273

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Rat Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Oxa Concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6 pg/ml. Sensitivity: 5 pg/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat OXA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

18490153

AAH22295.1

21,425 Da

Cell Surface Interactions At The Vascular Wall Pathway (106062); Hemostasis Pathway (106028); PPAR Signaling Pathway (83042); PPAR Signaling Pathway (450); Phagosome Pathway (153910); Phagosome Pathway (153859)

This gene encodes a low density lipoprotein receptor that belongs to the C-type lectin superfamily. This gene is regulated through the cyclic AMP signaling pathway. The encoded protein binds, internalizes and degrades oxidized low-density lipoprotein. This protein may be involved in the regulation of Fas-induced apoptosis. This protein may play a role as a scavenger receptor. Mutations of this gene have been associated with atherosclerosis, risk of myocardial infarction, and may modify the risk of Alzheimer's disease. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]

OLR1: Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro- oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro- atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram- positive bacteria. Independent association genetic studies have implicated OLR1 gene variants in myocardial infarction susceptibility. OLR1 may be involved in Alzheimer disease (AD). Involvement in AD is however unclear: according to some authors (PubMed:12354387, PubMed:12810610 and PubMed:15976314), variations in OLR1 modify the risk of AD, while according to other (PubMed:15000751 and PubMed:15060104) they do not. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Receptor, misc.; Membrane protein, integral. Chromosomal Location of Human Ortholog: 12p13.2-p12.3. Cellular Component: nucleoplasm; membrane; intracellular membrane-bound organelle; integral to plasma membrane; plasma membrane; receptor complex; lipid raft. Molecular Function: low-density lipoprotein receptor activity; protein binding; carbohydrate binding. Biological Process: cell death; receptor-mediated endocytosis; leukocyte adhesion; response to hydrogen peroxide; blood circulation; lipoprotein metabolic process; proteolysis; inflammatory response; blood coagulation; leukocyte migration. Disease: Myocardial Infarction, Susceptibility To

48-Strip-Wells

260 USD

96-Strip-Wells

405

5x96-Strip-Wells

1615

10x96-Strip-Wells

2880