Mouse Prostaglandin D2-methoxime (PGD2-MOX) ELISA Kit | MyBioSource MBS262231 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Prostaglandin D2-methoxime (PGD2-MOX) ELISA Kit

catalog :

MBS262231

quantity :

48-Strip-Wells

price :

290 USD

more info or order :

MyBioSource product webpage

citations: 1

Reference
Pettersson H, Zarnegar B, Westin A, Persson V, Peuckert C, Jönsson J, et al. SLC10A4 regulates IgE-mediated mast cell degranulation in vitro and mast cell-mediated reactions in vivo. Sci Rep. 2017;7:1085 pubmed publisher

product information

catalog number :

MBS262231

products type :

ELISA Kit

products full name :

Mouse Prostaglandin D2-methoxime (PGD2-MOX) ELISA Kit

products short name :

Prostaglandin D2-methoxime (PGD2-MOX)

other names :

prostaglandin D2 receptor 2; Prostaglandin D2 receptor 2; prostaglandin D2 receptor 2; G protein-coupled receptor 44; G-protein coupled receptor 44; putative G-protein coupled receptor 44; chemoattractant receptor-homologous molecule expressed on TH2 cells; chemoattractant receptor homologous molecule expressed on T helper type 2 cells; prostaglandin D2 receptor 2; Chemoattractant receptor-homologous molecule expressed on Th2 cells; G-protein coupled receptor 44; CD_antigen: CD294

products gene name :

PGD2-MOX

other gene names :

PTGDR2; PTGDR2; DP2; DL1R; CD294; CRTH2; GPR44; CRTH2; DL1R; GPR44

uniprot entry name :

PD2R2_HUMAN

reactivity :

Mouse

sequence length :

395

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PGD2-MOX concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse PGD2-MOX monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

153791424

ncbi acc num :

NP_004769.2

ncbi gb acc num :

NM_004778.2

ncbi mol weight :

43,268 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Eicosanoid Ligand-binding Receptors Pathway (106370); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Prostanoid Ligand Receptors Pathway (106371); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)

ncbi summary :

This gene encodes a G-protein-coupled receptor that is preferentially expressed in CD4+ effector T helper 2 (Th2) cells. This protein is a prostaglandin D2 receptor that mediates the pro-inflammatory chemotaxis of eosinophils, basophils, and Th2 lymphocytes generated during allergic inflammation. Single nucleotide polymorphisms in the 3' UTR of this gene have been associated with asthma susceptibility.[provided by RefSeq, Mar 2011]

uniprot summary :

GPR44: Receptor for prostaglandin D2 (PGD2). Coupled to the G(i)-protein. Receptor activation may result in pertussis toxin- sensitive decreases in cAMP levels and Ca(2+) mobilization. PI3K signaling is also implicated in mediating PTGDR2 effects. PGD2 induced receptor internalization. CRTH2 internalization can be regulated by diverse kinases such as, PKC, PKA, ADRBK1/GRK2, GPRK5/GRK5 and GRK6. Receptor activation is responsible, at least in part, in immune regulation and allergic/inflammation responses. Belongs to the G-protein coupled receptor 1 family. Protein type: GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral. Chromosomal Location of Human Ortholog: 11q12-q13.3. Cellular Component: neuron projection; integral to plasma membrane; plasma membrane. Molecular Function: G-protein coupled receptor activity; neuropeptide binding; prostaglandin F receptor activity; prostaglandin J receptor activity; prostaglandin D receptor activity. Biological Process: G-protein signaling, coupled to cyclic nucleotide second messenger; synaptic transmission; G-protein coupled receptor protein signaling pathway; neuropeptide signaling pathway; positive regulation of G-protein coupled receptor protein signaling pathway; calcium-mediated signaling; immune response; G-protein signaling, adenylate cyclase inhibiting pathway; chemotaxis

size1 :

48-Strip-Wells

price1 :

290 USD

size2 :

96-Strip-Wells

price2 :

450

more info or order :

MyBioSource product webpage