Rat Endothelial Nitric Oxide Synthase (eNOS) ELISA Kit | MyBioSource MBS262105 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Endothelial Nitric Oxide Synthase (eNOS) ELISA Kit

catalog :

MBS262105

quantity :

48-Strip-Wells

price :

290 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS262105

products type :

ELISA Kit

products full name :

Rat Endothelial Nitric Oxide Synthase (eNOS) ELISA Kit

products short name :

Endothelial Nitric Oxide Synthase (eNOS)

other names :

endothelial nitric oxide synthase; Nitric oxide synthase, endothelial; nitric oxide synthase, endothelial; cNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS; nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta21; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 (endothelial cell); Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII

products gene name :

eNOS

other gene names :

NOS3; NOS3; eNOS; ECNOS; cNOS; eNOS; NOSIII

uniprot entry name :

NOS3_HUMAN

reactivity :

Rat

sequence length :

1203

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant eNOS concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.06 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

. Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat eNOS monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

825652

ncbi acc num :

CAA53950.1

uniprot acc num :

P29474

ncbi mol weight :

67,876 Da

ncbi pathways :

ACE Inhibitor Pathway (198763); AGE/RAGE Pathway (698754); Angiogenesis Pathway (198772); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Disease Pathway (530764); Endothelin Pathway (672453)

ncbi summary :

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

uniprot summary :

eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN. Protein type: EC 1.14.13.39; Oxidoreductase; Amino Acid Metabolism - arginine and proline. Chromosomal Location of Human Ortholog: 7q36. Cellular Component: Golgi membrane; cytoskeleton; cytoplasm; plasma membrane; caveola; nucleus; cytosol. Molecular Function: actin monomer binding; calmodulin binding; protein binding; FAD binding; NADPH-hemoprotein reductase activity; nitric-oxide synthase activity; FMN binding; iron ion binding; heme binding; NADP binding; cadmium ion binding. Biological Process: regulation of systemic arterial blood pressure by endothelin; negative regulation of potassium ion transport; negative regulation of muscle hyperplasia; positive regulation of vasodilation; nitric oxide metabolic process; negative regulation of cell proliferation; negative regulation of hydrolase activity; regulation of the force of heart contraction by chemical signal; regulation of blood pressure; lipopolysaccharide-mediated signaling pathway; angiogenesis; negative regulation of blood pressure; nitric oxide biosynthetic process; regulation of blood vessel size; mitochondrion organization and biogenesis; interaction with host; negative regulation of calcium ion transport; in utero embryonic development; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; ovulation from ovarian follicle; positive regulation of angiogenesis; response to heat; endothelial cell migration; arginine catabolic process; blood vessel remodeling; regulation of nitric-oxide synthase activity; blood coagulation; vascular endothelial growth factor receptor signaling pathway; nitric oxide mediated signal transduction; smooth muscle hyperplasia; lung development. Disease: Preeclampsia/eclampsia 1; Alzheimer Disease; Hypertension, Essential; Stroke, Ischemic

size1 :

48-Strip-Wells

price1 :

290 USD

size2 :

96-Strip-Wells

price2 :

450

more info or order :

MyBioSource product webpage