ELISA/assay

Human Chemerin (CHEM) ELISA Kit

MBS262025

48-Strip-Wells

275 USD

ELISA Kit

Human Chemerin (CHEM) ELISA Kit

Chemerin (CHEM)

chemokine-like receptor 1 isoform a; Chemokine-like receptor 1; chemokine-like receptor 1; chemerin receptor; resolvin E1 receptor; chemokine receptor-like 1; G-protein coupled receptor DEZ; G-protein coupled receptor ChemR23; orphan G-protein coupled receptor, Dez; chemokine-like receptor 1; G-protein coupled receptor ChemR23; G-protein coupled receptor DEZ

CHEM

CMKLR1; CMKLR1; DEZ; RVER1; ChemR23; CHEMERINR; CHEMR23; DEZ

CML1_HUMAN

Human

373

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant CHEM concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: Up to 0.05ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human CHEM monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

215272321

NP_001135817.1

NM_001142345.1

42,035 Da

GPCRs, Class A Rhodopsin-like Pathway (198886)

CMKLR1: Receptor for the chemoattractant adipokine chemerin/RARRES2 and for the omega-3 fatty acid derived molecule resolvin E1. Interaction with RARRES2 induces activation of intracellular signaling molecules, such as SKY, MAPK1/3 (ERK1/2), MAPK14/P38MAPK and PI3K leading to multifunctional effects, like, reduction of immune responses, enhancing of adipogenesis and angionesis. Resolvin E1 down-regulates cytokine production in macrophages by reducing the activation of MAPK1/3 (ERK1/2) and NF- kappa-B. Acts as a coreceptor for several SIV strains (SIVMAC316, SIVMAC239, SIVMACL7E-FR and SIVSM62A), as well as a primary HIV-1 strain (92UG024-2). Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR. Chromosomal Location of Human Ortholog: 12q24.1. Cellular Component: integral to plasma membrane; integral to membrane; plasma membrane. Molecular Function: G-protein coupled receptor activity; protein binding; chemokine receptor activity; receptor activity. Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of interleukin-12 production; inhibition of NF-kappaB transcription factor; regulation of calcium-mediated signaling; positive regulation of fat cell differentiation; immune response; chemotaxis; skeletal development

48-Strip-Wells

275 USD

96-Strip-Wells

420