Mouse Endothelial Nitric Oxide Synthase 3 (eNOS-3) ELISA Kit | MyBioSource MBS262021 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Endothelial Nitric Oxide Synthase 3 (eNOS-3) ELISA Kit

catalog :

MBS262021

quantity :

48-Strip-Wells

price :

290 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS262021

products type :

ELISA Kit

products full name :

Mouse Endothelial Nitric Oxide Synthase 3 (eNOS-3) ELISA Kit

products short name :

Endothelial Nitric Oxide Synthase 3 (eNOS-3)

other names :

endothelial nitric oxide synthase 3, partial; Endothelial nitric oxide synthase 3; nitric oxide synthase, endothelial; cNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS; endothelial nitric oxide synthase 3; nitric oxide synthase 3, endothelial cell; Endothelial nitric oxide synthase 3

products gene name :

eNOS-3

other gene names :

Nos3; nos3; eNos

uniprot entry name :

Q9JJY7_RAT

reactivity :

Mouse

sequence length :

306

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant eNOS-3 concentration. Assay Type: Sandwich. Detection Range: 100 U/ml-1.56 U/ml. Sensitivity: 0.5 U/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse eNOS-3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

7259222

ncbi acc num :

CAB77547.1

uniprot acc num :

Q9JJY7

ncbi mol weight :

33,804 Da

ncbi pathways :

ACE Inhibitor Pathway (198448); Arginine And Proline Metabolism Pathway (83349); Arginine And Proline Metabolism Pathway (323); Calcium Signaling Pathway (83442); Calcium Signaling Pathway (459); Disease Pathway (828366); Estrogen Signaling Pathway (799190); Estrogen Signaling Pathway (799197); HIF-1 Signaling Pathway (695233); Hemostasis Pathway (828446)

ncbi summary :

enzyme that synthesizes Nitric oxide from L-arginine [RGD, Feb 2006]

uniprot summary :

eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN. Protein type: EC 1.14.13.39; Oxidoreductase; Amino Acid Metabolism - arginine and proline. Cellular Component: Golgi apparatus; cytoskeleton; mitochondrion; apical part of cell; cytoplasm; nucleolus; plasma membrane; vesicle membrane; caveola; cytosol; nucleus; sarcolemma; lipid raft. Molecular Function: actin monomer binding; FAD binding; nitric-oxide synthase activity; beta-catenin binding; Hsp90 protein binding; actin binding; calmodulin binding; protein binding; NADPH-hemoprotein reductase activity; FMN binding; cadherin binding; iron ion binding; heme binding; nitric-oxide synthase binding; NADP binding. Biological Process: response to peptide hormone stimulus; response to metal ion; regulation of systemic arterial blood pressure by endothelin; negative regulation of smooth muscle cell proliferation; positive regulation of apoptosis; negative regulation of potassium ion transport; negative regulation of muscle hyperplasia; response to lipopolysaccharide; female pregnancy; positive regulation of vasodilation; signal transduction; response to organic cyclic substance; response to estradiol stimulus; negative regulation of cell proliferation; negative regulation of hydrolase activity; regulation of the force of heart contraction by chemical signal; lipopolysaccharide-mediated signaling pathway; response to lipoprotein stimulus; angiogenesis; negative regulation of blood pressure; response to axon injury; nitric oxide biosynthetic process; regulation of blood vessel size; response to fructose stimulus; aging; response to corticosterone stimulus; response to drug; negative regulation of calcium ion transport; in utero embryonic development; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; ovulation from ovarian follicle; response to amino acid stimulus; positive regulation of angiogenesis; response to ethanol; response to hyperoxia; retina development in camera-type eye; response to mechanical stimulus; response to cytokine stimulus; response to lead ion; response to hypoxia; arginine catabolic process; endothelial cell migration; blood vessel remodeling; response to activity; nitric oxide mediated signal transduction; smooth muscle hyperplasia; lung development

size1 :

48-Strip-Wells

price1 :

290 USD

size2 :

96-Strip-Wells

price2 :

450

more info or order :

MyBioSource product webpage