ELISA/assay

Mouse Interleukin 5 (IL-5) ELISA Kit

MBS262012

48-Strip-Wells

290 USD

ELISA Kit

Mouse Interleukin 5 (IL-5) ELISA Kit

Interleukin 5 (IL-5)

interleukin-5; Interleukin-5; interleukin-5; T-cell replacing factor; B-cell differentiation factor I; eosinophil differentiation factor; colony-stimulating factor, eosinophil; interleukin 5; B-cell differentiation factor I; Eosinophil differentiation factor; T-cell replacing factor; TRF

IL-5

IL5; IL5; EDF; TRF; IL-5; IL-5; TRF

IL5_HUMAN

Mouse

134

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant IL-5 concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6 pg/ml. Sensitivity: 5 pg/ml.

Intra-assay Precision: ? 8%. Inter-assay Precision: ? 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse IL-5 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

4504671

NP_000870.1

NM_000879.2

15,238 Da

Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Asthma Pathway (83120); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460)

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]

IL5: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells. Belongs to the IL-5 family. Protein type: Secreted, signal peptide; Cytokine; Secreted. Chromosomal Location of Human Ortholog: 5q31.1. Cellular Component: extracellular space; extracellular region. Molecular Function: interleukin-5 receptor binding; protein binding; growth factor activity; cytokine activity. Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of B cell proliferation; positive regulation of JAK-STAT cascade; positive regulation of transcription factor activity; immune response; positive regulation of immunoglobulin secretion; inflammatory response; positive regulation of eosinophil differentiation

48-Strip-Wells

290 USD

96-Strip-Wells

450