Human Adenosine Deaminase (ADA) ELISA Kit | MyBioSource MBS261964 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Adenosine Deaminase (ADA) ELISA Kit

catalog :

MBS261964

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS261964

products type :

ELISA Kit

products full name :

Human Adenosine Deaminase (ADA) ELISA Kit

products short name :

Adenosine Deaminase (ADA)

other names :

adenosine deaminase; Adenosine deaminase; adenosine deaminase; adenosine aminohydrolase; adenosine deaminase; Adenosine aminohydrolase

products gene name :

ADA

other gene names :

ADA; ADA; ADA1

uniprot entry name :

ADA_HUMAN

reactivity :

Human

sequence length :

363

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant ADA concentration. Assay Type: Sandwich. Detection Range: 100 ng/ml-1.56 ng/ml. Sensitivity: 0.5ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human ADA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

47078295

ncbi acc num :

NP_000013.2

ncbi gb acc num :

NM_000022.2

ncbi mol weight :

40,764 Da

ncbi pathways :

C-MYB Transcription Factor Network Pathway (138073); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Primary Immunodeficiency Pathway (83125); Primary Immunodeficiency Pathway (537); Purine Metabolism Pathway (82944); Purine Metabolism Pathway (106265); Purine Metabolism Pathway (307); Purine Salvage Pathway (106273); Adenine And Adenosine Salvage III Pathway (547495)

ncbi summary :

This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia. [provided by RefSeq, Jul 2008]

uniprot summary :

ADA: a enzyme that converts adenosine + H2O into inosine + NH3. Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues. Genetic ADA deficiencies are a cause of autosomal recessive severe combined immuno-deficiency (SCID). Hereditary hemolytic anemia is caused by expression levels in erythrocytes 50-70 times greater than the norm. Protein type: Hydrolase; Nucleotide Metabolism - purine; EC 3.5.4.4. Chromosomal Location of Human Ortholog: 20q13.12. Cellular Component: dendrite cytoplasm; extracellular space; cell surface; cell soma; membrane; lysosome; cytoplasm; plasma membrane; cell junction; cytosol; external side of plasma membrane. Molecular Function: adenosine deaminase activity; protein binding; zinc ion binding; purine nucleoside binding. Biological Process: negative regulation of circadian sleep/wake cycle, non-REM sleep; T cell activation; adenosine catabolic process; deoxyadenosine catabolic process; response to morphine; positive regulation of calcium-mediated signaling; purine salvage; positive regulation of T cell differentiation in the thymus; histamine secretion; purine ribonucleoside monophosphate biosynthetic process; regulation of cell-cell adhesion mediated by integrin; response to vitamin E; negative regulation of mature B cell apoptosis; positive regulation of T cell receptor signaling pathway; positive regulation of B cell proliferation; positive regulation of germinal center formation; hypoxanthine salvage; negative regulation of adenosine receptor signaling pathway; positive regulation of smooth muscle contraction; embryonic gut development; placenta development; aging; Peyer s patch development; response to drug; dATP catabolic process; nucleobase, nucleoside and nucleotide metabolic process; positive regulation of heart rate; liver development; negative regulation of leukocyte migration; purine base metabolic process; trophectodermal cell differentiation; purine nucleotide salvage; response to hydrogen peroxide; xanthine biosynthetic process; negative regulation of inflammatory response; response to hypoxia; inosine biosynthetic process; germinal center B cell differentiation; alveolus development; positive regulation of alpha-beta T cell differentiation. Disease: Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-negative, Nk Cell-negative, Due To Adenosine Deaminase Deficiency

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

420

more info or order :

MyBioSource product webpage