Chicken Interleukin 12 (IL-12) ELISA Kit | MyBioSource MBS261949 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Chicken Interleukin 12 (IL-12) ELISA Kit

catalog :

MBS261949

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS261949

products type :

ELISA Kit

products full name :

Chicken Interleukin 12 (IL-12) ELISA Kit

products short name :

[Interleukin 12 (IL-12)]

other names :

[interleukin 12; Interleukin-12 subunit alpha; interleukin-12 subunit alpha; CLMF p35; IL35 subunit; IL-12, subunit p35; interleukin 12, p35; interleukin-12 alpha chain; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin 12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; NK cell stimulatory factor chain 1; NKSF1]

products gene name :

[IL-12]

other gene names :

[IL12A; IL12A; P35; CLMF; NFSK; NKSF1; IL-12A; NKSF1; IL-12A; CLMF p35; NKSF1]

uniprot entry name :

IL12A_HUMAN

reactivity :

Chicken

sequence length :

253

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Chicken Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Igm Concentration. Assay Type: Sandwich. Detection Range: 1000 ng/ml-15.6 ng/ml. Sensitivity: 5 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Chicken IgM monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

4323579

ncbi acc num :

AAD16432.1

ncbi mol weight :

24,874 Da

ncbi pathways :

African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460)

ncbi summary :

This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]

uniprot summary :

IL12A: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. Belongs to the IL-6 superfamily. Protein type: Cytokine; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 3q25.33. Cellular Component: extracellular space; interleukin-12 complex; cytoplasm. Molecular Function: interleukin-27 binding; protein binding; growth factor activity; interleukin-12 beta subunit binding; protein heterodimerization activity; cytokine activity; interleukin-12 receptor binding. Biological Process: cell migration; positive regulation of NK T cell activation; positive regulation of cell adhesion; positive regulation of T cell mediated cytotoxicity; negative regulation of smooth muscle cell proliferation; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; response to virus; positive regulation of tyrosine phosphorylation of Stat4 protein; response to lipopolysaccharide; positive regulation of natural killer cell mediated cytotoxicity; defense response to protozoan; positive regulation of natural killer cell activation; response to UV-B; defense response to Gram-positive bacterium; positive regulation of interferon-gamma production; negative regulation of interleukin-17 production; positive regulation of T cell differentiation; positive regulation of mononuclear cell proliferation; positive regulation of lymphocyte proliferation; positive regulation of T cell proliferation; immune response; cell cycle arrest

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

435

size3 :

5x96-Strip-Wells

price3 :

1755

size4 :

10x96-Strip-Wells

price4 :

3150

more info or order :

MyBioSource product webpage