ELISA/assay

Human Glycogen phosphorylase BB (GP-BB) ELISA Kit

MBS261920

48-Strip-Wells

275 USD

ELISA Kit

Human Glycogen phosphorylase BB (GP-BB) ELISA Kit

Glycogen phosphorylase BB (GP-BB)

glycogen phosphorylase, brain form; Glycogen phosphorylase, brain form; glycogen phosphorylase, brain form; glycogen phosphorylase B; phosphorylase, glycogen; brain

GP-BB

PYGB; PYGB; GPBB

PYGB_HUMAN

Human

843

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant GP-BB concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human GP-BB monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

21361370

NP_002853.2

NM_002862.3

96,696 Da

Disease Pathway (530764); Glucose Metabolism Pathway (106199); Glycogen Metabolism Pathway (198856); Glycogen Breakdown (glycogenolysis) Pathway (106212); Glycogen Storage Diseases Pathway (980468); Insulin Signaling Pathway (83090); Insulin Signaling Pathway (501); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Carbohydrates Pathway (106196)

The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation. [provided by RefSeq, Jul 2008]

PYGB: Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Belongs to the glycogen phosphorylase family. Protein type: Carbohydrate Metabolism - starch and sucrose; Transferase; EC 2.4.1.1; Kinase, other; Phosphorylase. Chromosomal Location of Human Ortholog: 20p11.21. Cellular Component: membrane; cytoplasm. Molecular Function: glycogen phosphorylase activity; pyridoxal phosphate binding. Biological Process: glycogen catabolic process; carbohydrate metabolic process; glucose metabolic process; pathogenesis

48-Strip-Wells

275 USD

96-Strip-Wells

420