ELISA/assay

Chicken Gastric Inhibitory Polypeptide (GIP) ELISA Kit

MBS261884

48-Strip-Wells

315 USD

ELISA Kit

Chicken Gastric Inhibitory Polypeptide (GIP) ELISA Kit

Gastric Inhibitory Polypeptide (GIP)

gastric inhibitory polypeptide preproprotein; Gastric inhibitory polypeptide; gastric inhibitory polypeptide; incretin hormone; glucose-dependent insulinotropic polypeptide; gastric inhibitory polypeptide; Glucose-dependent insulinotropic polypeptide; Incretin hormone

GIP

GIP; GIP; GIP

GIP_HUMAN

Chicken

153

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Chicken serum, plasma or cell culture supernatant and organizations in the natural and recombinant GIP concentration. Assay Type: Sandwich. Detection Range: 5000 pg/ml-78 pg/ml. Sensitivity: 20 pg/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Chicken GIP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

4758436

NP_004114.1

NM_004123.2

17,108 Da

Class B/2 (Secretin Family Receptors) Pathway (106378); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Glucagon-type Ligand Receptors Pathway (106380); Incretin Synthesis, Secretion, And Inactivation Pathway (187170); Insulin Secretion Pathway (777534); Metabolism Of Proteins Pathway (106230); Peptide Hormone Metabolism Pathway (771603); Signal Transduction Pathway (477114)

This gene encodes an incretin hormone and belongs to the glucagon superfamily. The encoded protein is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nutrient absorption. This gene stimulates insulin secretion via its G protein-coupled receptor activation of adenylyl cyclase and other signal transduction pathways. It is a relatively poor inhibitor of gastric acid secretion. [provided by RefSeq, Jul 2008]

GIP: Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion. Belongs to the glucagon family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 17q21.3-q22. Cellular Component: extracellular space; cell soma; endoplasmic reticulum lumen; extracellular region. Molecular Function: hormone activity. Biological Process: response to drug; response to peptide hormone stimulus; adult locomotory behavior; positive regulation of insulin secretion; female pregnancy; sensory perception of pain; signal transduction; response to lipid; response to amino acid stimulus; response to organic cyclic substance; endocrine pancreas development; memory; response to starvation; response to selenium ion; cellular protein metabolic process; response to glucose stimulus; response to axon injury; regulation of insulin secretion

48-Strip-Wells

315 USD

96-Strip-Wells

490