Human leukocyte antigen E (HLA-E) ELISA Kit | MyBioSource MBS261830 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human leukocyte antigen E (HLA-E) ELISA Kit

catalog :

MBS261830

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS261830

products type :

ELISA Kit

products full name :

Human leukocyte antigen E (HLA-E) ELISA Kit

products short name :

leukocyte antigen E (HLA-E)

other names :

leukocyte antigen F; HLA class I histocompatibility antigen, alpha chain F; HLA class I histocompatibility antigen, alpha chain F; HLA F antigen; leukocyte antigen F; HLA class I molecule; MHC class Ib antigen; MHC class I antigen F; major histocompatibility complex, class I, F; CDA12; HLA F antigen; Leukocyte antigen F; MHC class I antigen F

products gene name :

HLA-E

other gene names :

HLA-F; HLA-F; HLAF; CDA12; HLA-5.4; HLA-CDA12; HLA-5.4; HLAF

uniprot entry name :

HLAF_HUMAN

reactivity :

Human

sequence length :

362

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant HLA-E concentration. Assay Type: Sandwich. Detection Range: 200 U/ml-3.12 U/ml. Sensitivity: 0.6 U/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human HLA-E monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

312407

ncbi acc num :

CAA34947.1

uniprot acc num :

P30511

ncbi mol weight :

50,438 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway (366163); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Antigen Processing-Cross Presentation Pathway (477122); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533)

ncbi summary :

This gene belongs to the HLA class I heavy chain paralogues. It encodes a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation. This gene exhibits few polymorphisms. Multiple transcript variants encoding different isoforms have been found for this gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain. [provided by RefSeq, Jul 2008]

uniprot summary :

HLA-F: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: Golgi membrane; phagocytic vesicle membrane; cell surface; membrane; endoplasmic reticulum; early endosome membrane; plasma membrane; MHC class I protein complex. Molecular Function: TAP1 binding; peptide antigen binding; TAP2 binding; receptor binding. Biological Process: antigen processing and presentation of peptide antigen via MHC class I; regulation of immune response; positive regulation of T cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

420

more info or order :

MyBioSource product webpage