catalog number :
MBS261790
products type :
ELISA Kit
products full name :
Rat Nerve Growth Factor (NGF) ELISA kit
products short name :
Nerve Growth Factor (NGF)
other names :
nerve growth factor; Nerve growth factor; nerve growth factor; zNGF; nerve growth factor (beta polypeptide)
other gene names :
ngf; ngf; ngfb; ns:zft386; xx:zft386; zgc:109730; ngfb; NGF; zNGF
uniprot entry name :
NGF_DANRE
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant NGF concentration. Assay Type: Sandwich. Detection Range: 2000 pg/ml-31.2 pg/ml. Sensitivity: 12 pg/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
. Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat NGF monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_954680.1
ncbi gb acc num :
NM_199210.1
ncbi mol weight :
21,903 Da
ncbi pathways :
ARMS-mediated Activation Pathway (823191); Activation Of TRKA Receptors Pathway (823182); Apoptosis Pathway (83637); Apoptosis Pathway (470); Axonal Growth Stimulation Pathway (823178); Cell Death Signalling Via NRAGE, NRIF And NADE Pathway (823169); Ceramide Signalling Pathway (823176); Frs2-mediated Activation Pathway (823190); MAPK Signaling Pathway (83627); MAPK Signaling Pathway (456)