Guinea pig Prekallikrein (PK) ELISA Kit | MyBioSource MBS261688 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Guinea pig Prekallikrein (PK) ELISA Kit

catalog :

MBS261688

quantity :

48-Strip-Wells

price :

315 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS261688

products type :

ELISA Kit

products full name :

Guinea pig Prekallikrein (PK) ELISA Kit

products short name :

Prekallikrein (PK)

other names :

plasma kallikrein preproprotein; Plasma kallikrein; plasma kallikrein; kininogenin; Fletcher factor; plasma prekallikrein; plasma kallikrein heavy chain; plasma kallikrein light chain; kallikrein B, plasma (Fletcher factor) 1; Fletcher factor; Kininogenin; Plasma prekallikreinCleaved into the following 2 chains:Plasma kallikrein heavy chain; Plasma kallikrein light chain

products gene name :

other gene names :

KLKB1; KLKB1; PPK; KLK3; KLK3

uniprot entry name :

KLKB1_HUMAN

reactivity :

Guinea Pig

sequence length :

638

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Guinea pig serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PK concentration. Assay Type: Sandwich. Detection Range: 100 ng/ml-1.56 ng/ml. Sensitivity: 0.5 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Guinea pig PK monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

78191798

ncbi acc num :

NP_000883.2

ncbi gb acc num :

NM_000892.3

ncbi mol weight :

71,370 Da

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (576264); Blood Clotting Cascade Pathway (198840); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057); Hemostasis Pathway (106028); Intrinsic Pathway (106059)

ncbi summary :

Plasma prekallikrein is a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. It is synthesized in the liver and secreted into the blood as a single polypeptide chain. Plasma prekallikrein is converted to plasma kallikrein by factor XIIa by the cleavage of an internal Arg-Ile bond. Plasma kallikrein therefore is composed of a heavy chain and a light chain held together by a disulphide bond. The heavy chain originates from the amino-terminal end of the zymogen and contains 4 tandem repeats of 90 or 91 amino acids. Each repeat harbors a novel structure called the apple domain. The heavy chain is required for the surface-dependent pro-coagulant activity of plasma kallikrein. The light chain contains the active site or catalytic domain of the enzyme and is homologous to the trypsin family of serine proteases. Plasma prekallikrein deficiency causes a prolonged activated partial thromboplastin time in patients. [provided by RefSeq, Jul 2008]

uniprot summary :

KLKB1: The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin. Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency); also known as Fletcher factor deficiency. This disorder is a blood coagulation defect. Belongs to the peptidase S1 family. Plasma kallikrein subfamily. Protein type: Secreted, signal peptide; Protease; EC 3.4.21.34; Secreted. Chromosomal Location of Human Ortholog: 4q35. Cellular Component: extracellular space; plasma membrane; extracellular region. Molecular Function: protein binding; serine-type endopeptidase activity. Biological Process: extracellular matrix disassembly; fibrinolysis; extracellular matrix organization and biogenesis; positive regulation of fibrinolysis; zymogen activation; blood coagulation; proteolysis; blood coagulation, intrinsic pathway; plasminogen activation; Factor XII activation. Disease: Prekallikrein Deficiency

size1 :

48-Strip-Wells

price1 :

315 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage