ELISA/assay

Human 5-lipoxygenase (5-LO) ELISA Kit

MBS261618

48-Strip-Wells

275 USD

ELISA Kit

Human 5-lipoxygenase (5-LO) ELISA Kit

5-lipoxygenase (5-LO)

5-lipoxygenase, partial; Arachidonate 5-lipoxygenase; arachidonate 5-lipoxygenase; LOX-5; leukotriene A4 synthase; arachidonic acid 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform; arachidonate 5-lipoxygenase

5-LO

ALOX5; ALOX5; 5-LO; 5LPG; LOG5; 5-LOX; LOG5; 5-LO; 5-lipoxygenase

LOX5_HUMAN

Human

50

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant 5-LO concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: Up to 0.05 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human 5-LO monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

187167

AAA63212.1

56,291 Da

Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (685553); Arachidonic Acid Metabolism Pathway (366); Eicosanoid Synthesis Pathway (198888); IL-5 Signaling Pathway (198770); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Ovarian Steroidogenesis Pathway (790011); Ovarian Steroidogenesis Pathway (795173); Selenium Pathway (198825)

This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

5-LO: arachidonate 5-lipoxygenase. Arachidonic acid promotes its phosphorylation by p38 MAPK-regulated MAPKAP kinases. Upregulated in glioblastoma multiforme and colon, pancreatic and bladder cancer. Protein type: Lipid Metabolism - linoleic acid; Lipid Metabolism - arachidonic acid; Oxidoreductase; Nuclear envelope; EC 1.13.11.34. Chromosomal Location of Human Ortholog: 10q11.2. Cellular Component: extracellular space; nuclear membrane; nuclear matrix; nuclear envelope lumen; nuclear envelope; cytosol. Molecular Function: protein binding; iron ion binding; arachidonate 5-lipoxygenase activity. Biological Process: leukotriene biosynthetic process; leukotriene production during acute inflammatory response; lipoxygenase pathway; arachidonic acid metabolic process; leukotriene metabolic process. Disease: Asthma, Susceptibility To

48-Strip-Wells

275 USD

96-Strip-Wells

420