catalog number :
MBS261563
products type :
ELISA Kit
products full name :
Porcine Aspartate aminotransferase (AST) ELISA Kit
products short name :
[Aspartate aminotransferase (AST)]
other names :
[aspartate aminotransferase; Aspartate aminotransferase, chloroplastic; aspartate aminotransferase; Transaminase A]
products gene name :
[AST]
other gene names :
[ASP5; ASP5; AAT3; ASP5; ASPARTATE AMINOTRANSFERASE; aspartate aminotransferase 5; ASPARTATE AMINOTRANSFERASE DEFICIENT 3; ATAAT1; F11C18.15; AAT1; At4g31990; F10N7.200]
uniprot entry name :
AAT5_ARATH
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Porcine Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Ang-1 Concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine ANG-1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_001190885.1
ncbi gb acc num :
NM_001203956.1
ncbi mol weight :
49,831 Da
ncbi pathways :
2-Oxocarboxylic Acid Metabolism Pathway (714629); 2-Oxocarboxylic Acid Metabolism Pathway (717400); Alanine, Aspartate And Glutamate Metabolism Pathway (100103); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Amino Acid Synthesis And Interconversion (transamination) Pathway (826107); Arginine And Proline Metabolism Pathway (4017); Arginine And Proline Metabolism Pathway (323); Biosynthesis Of Amino Acids Pathway (790118); Biosynthesis Of Amino Acids Pathway (795174); Cysteine And Methionine Metabolism Pathway (103454)
ncbi summary :
encodes a plastid-localized aspartate aminotransferase
uniprot summary :
Amino acid aminotransferase important for the metabolism of amino acids and Krebs-cycle related organic acids. No activity with D-Asp or D-Ala as amino donors. In plants, it is involved in nitrogen metabolism and in aspects of carbon and energy metabolism.
size4 :
10x96-Strip-Wells