catalog number :
MBS261495
products type :
ELISA Kit
products full name :
Human Protamine 1 (PRM1) ELISA Kit
products short name :
Protamine 1 (PRM1)
other names :
protamine 1; Sperm protamine P1; sperm protamine P1; cysteine-rich protamine; testis specific protamine 1; cancer/testis antigen family 94, member 1; protamine 1; Cysteine-rich protamine
products gene name :
PRM1
other gene names :
PRM1; PRM1; P1; CT94.1
uniprot entry name :
HSP1_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PRM1 concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human PRM1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
EAW85157.1
ncbi mol weight :
6,823 Da
uniprot summary :
PRM1: Protamines substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex. Belongs to the protamine P1 family. Protein type: Cancer Testis Antigen (CTA); DNA-binding. Chromosomal Location of Human Ortholog: 16p13.2. Cellular Component: nucleoplasm; nucleosome. Molecular Function: DNA binding. Biological Process: chromosome condensation; multicellular organismal development; spermatogenesis; cell differentiation; DNA packaging