catalog number :
MBS261374
products type :
ELISA Kit
products full name :
Rat Brain Derived Neurotrophic Factor (BDNF) ELISA Kit
products short name :
Brain Derived Neurotrophic Factor (BDNF)
other names :
brain derived neurotrophic factor; Brain-derived neurotrophic factor; brain-derived neurotrophic factor; pre-pro-brain-derived; brain-derived neurotrophic factor
products gene name :
BDNF
other gene names :
BDNF; BDNF; BDNF
uniprot entry name :
BDNF_CHICK
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant BDNF concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat BDNF monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
AAZ31074.1
ncbi mol weight :
27,715 Da
ncbi pathways :
MAPK Signaling Pathway (199082); MAPK Signaling Pathway (83956); MAPK Signaling Pathway (456)