ELISA/assay

Guinea pig Renin (REN) ELISA Kit

MBS261292

48-Strip-Wells

315 USD

ELISA Kit

Guinea pig Renin (REN) ELISA Kit

Renin (REN)

renin preproprotein; Renin; renin; angiotensinogenase; renin precursor, renal; angiotensin-forming enzyme; renin; Angiotensinogenase

REN

REN; REN; HNFJ2

RENI_HUMAN

Guinea Pig

406

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Guinea pig serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant REN concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6pg/ml. Sensitivity: 5 pg/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Guinea pig REN monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

4506475

NP_000528.1

NM_000537.3

44,726 Da

ACE Inhibitor Pathway (198763); Metabolism Of Angiotensinogen To Angiotensins Pathway (685555); Metabolism Of Proteins Pathway (106230); Peptide Hormone Metabolism Pathway (771603); Renin-angiotensin System Pathway (83075); Renin-angiotensin System Pathway (486)

Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia. [provided by RefSeq, Jul 2008]

REN: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Interacts with ATP6AP2. Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide; Protease; EC 3.4.23.15. Chromosomal Location of Human Ortholog: 1q32. Cellular Component: extracellular space; cytoplasm; extracellular region; plasma membrane. Molecular Function: peptidase activity; insulin-like growth factor receptor binding; aspartic-type endopeptidase activity; receptor binding. Biological Process: response to cAMP; hormone-mediated signaling; male gonad development; renin-angiotensin regulation of aldosterone production; drinking behavior; cell maturation; response to lipopolysaccharide; angiotensin maturation; proteolysis; cellular protein metabolic process; regulation of MAPKKK cascade; beta-amyloid metabolic process; regulation of blood pressure; mesonephros development; kidney development. Disease: Renal Tubular Dysgenesis; Hyperuricemic Nephropathy, Familial Juvenile, 2

48-Strip-Wells

315 USD

96-Strip-Wells

490