Rat Thioredoxin Reductase 1 (TrxR1) ELISA Kit | MyBioSource MBS261228 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Thioredoxin Reductase 1 (TrxR1) ELISA Kit

catalog :

MBS261228

quantity :

48-Strip-Wells

price :

290 USD

more info or order :

MyBioSource product webpage

citations: 1

Reference
Ruszkiewicz J, Albrecht J. Changes of the thioredoxin system, glutathione peroxidase activity and total antioxidant capacity in rat brain cortex during acute liver failure: modulation by L-histidine. Neurochem Res. 2015;40:293-300 pubmed publisher

product information

catalog number :

MBS261228

products type :

ELISA Kit

products full name :

Rat Thioredoxin Reductase 1 (TrxR1) ELISA Kit

products short name :

Thioredoxin Reductase 1 (TrxR1)

other names :

thioredoxin reductase 1; Thioredoxin reductase 1, cytoplasmic; thioredoxin reductase 1, cytoplasmic; oxidoreductase; thioredoxin reductase TR1; thioredoxin reductase GRIM-12; KM-102-derived reductase-like factor; gene associated with retinoic and IFN-induced mortality 12 protein; gene associated with retinoic and interferon-induced mortality 12 protein; thioredoxin reductase 1; Gene associated with retinoic and interferon-induced mortality 12 protein; GRIM-12; Gene associated with retinoic and IFN-induced mortality 12 protein; KM-102-derived reductase-like factor; Thioredoxin reductase TR1

products gene name :

TrxR1

other gene names :

TXNRD1; TXNRD1; TR; TR1; TXNR; TRXR1; GRIM-12; GRIM12; KDRF; TR; GRIM-12; Gene associated with retinoic and IFN-induced mortality 12 protein

uniprot entry name :

TRXR1_HUMAN

reactivity :

Rat

sequence length :

647

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant TrxR1 concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.

other info2 :

Intra-assay Precision: ? 8%. Inter-assay Precision: ? 12%

products description :

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat TrxR1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

37542493

ncbi acc num :

AAL15432.1

ncbi mol weight :

50,873 Da

ncbi pathways :

Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Metabolism Of Nucleotides Pathway (106263); Oxidative Stress Pathway (198916); PPARA Activates Gene Expression Pathway (530749); Pyrimidine Metabolism Pathway (82946); Pyrimidine Metabolism Pathway (309); Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway (119544); Selenium Pathway (219762)

ncbi summary :

This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms. [provided by RefSeq, Jul 2008]

uniprot summary :

TRXR1: a cytoplasmic pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine residue which is required for the catalytic activity. Protein type: EC 1.8.1.9; Nuclear receptor co-regulator; Oxidoreductase; Nucleotide Metabolism - pyrimidine. Chromosomal Location of Human Ortholog: 12q23-q24.1. Cellular Component: nucleoplasm; nucleolus; cytosol. Molecular Function: protein binding; thioredoxin-disulfide reductase activity; electron carrier activity; FAD binding; protein disulfide oxidoreductase activity. Biological Process: response to reactive oxygen species; nucleobase, nucleoside and nucleotide interconversion; cell redox homeostasis; nucleobase, nucleoside and nucleotide metabolic process; cellular lipid metabolic process; signal transduction

size1 :

48-Strip-Wells

price1 :

290 USD

size2 :

96-Strip-Wells

price2 :

450

more info or order :

MyBioSource product webpage