Mouse S100 Calcium Binding Protein B (S100B) ELISA Kit | MyBioSource MBS261174 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse S100 Calcium Binding Protein B (S100B) ELISA Kit

catalog :

MBS261174

quantity :

48-Strip-Wells

price :

290 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS261174

products type :

ELISA Kit

products full name :

Mouse S100 Calcium Binding Protein B (S100B) ELISA Kit

products short name :

S100 Calcium Binding Protein B (S100B)

other names :

S100 calcium binding protein B; Protein S100-B; protein S100-B; S-100 protein subunit beta; S-100 calcium-binding protein, beta chain; S100 calcium-binding protein, beta (neural); S100 calcium binding protein B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B

products gene name :

S100B

other gene names :

S100B; S100B; NEF; S100; S100-B; S100beta

uniprot entry name :

S100B_HUMAN

reactivity :

Mouse

sequence length :

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Mouse serum, plasma or cell culture supernatant and organizations in the natural and recombinant S100B concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6 pg/ml. Sensitivity: 5 pg/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse S100B monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

12804681

ncbi acc num :

AAH01766.1

ncbi mol weight :

10,713 Da

ncbi pathways :

Activated TLR4 Signalling Pathway (106400); Advanced Glycosylation Endproduct Receptor Signaling Pathway (187092); Cytosolic Sensors Of Pathogen-associated DNA Pathway (576255); DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway (833822); Immune System Pathway (106386); Innate Immune System Pathway (106387); MyD88 Cascade Initiated On Plasma Membrane Pathway (205107); MyD88 Dependent Cascade Initiated On Endosome Pathway (187081); MyD88-independent Cascade Pathway (106401); MyD88:Mal Cascade Initiated On Plasma Membrane Pathway (106390)

ncbi summary :

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008]

uniprot summary :

S100B: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38. Interacts with AGER. The S100B dimer interacts with two molecules of CAPZA1. Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. Belongs to the S-101 family. Protein type: Calcium-binding. Chromosomal Location of Human Ortholog: 21q22.3. Cellular Component: ruffle; extracellular space; cell soma; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; extracellular region; nucleus. Molecular Function: identical protein binding; RAGE receptor binding; protein binding; protein homodimerization activity; zinc ion binding; calcium ion binding; tau protein binding; calcium-dependent protein binding. Biological Process: central nervous system development; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of apoptosis; response to glucocorticoid stimulus; memory; regulation of cell shape; cell proliferation; learning and/or memory; axonogenesis; astrocyte differentiation; positive regulation of cell proliferation; response to methylmercury; innate immune response; regulation of neuronal synaptic plasticity

size1 :

48-Strip-Wells

price1 :

290 USD

size2 :

96-Strip-Wells

price2 :

450

more info or order :

MyBioSource product webpage