Rat Aggrecan (AGC) ELISA Kit | MyBioSource MBS261073 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Aggrecan (AGC) ELISA Kit

catalog :

MBS261073

quantity :

48-Strip-Wells

price :

290 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS261073

products type :

ELISA Kit

products full name :

Rat Aggrecan (AGC) ELISA Kit

products short name :

Aggrecan (AGC)

other names :

Aggrecan; Aggrecan core protein; aggrecan core protein; large aggregating proteoglycan; cartilage-specific proteoglycan core protein; chondroitin sulfate proteoglycan core protein 1; aggrecan; Cartilage-specific proteoglycan core protein; CSPCP; Chondroitin sulfate proteoglycan core protein 1; Chondroitin sulfate proteoglycan 1

products gene name :

AGC

other gene names :

ACAN; ACAN; AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP; AGC1; CSPG1; MSK16; CSPCP; Chondroitin sulfate proteoglycan 1

uniprot entry name :

PGCA_HUMAN

reactivity :

Rat

sequence length :

721

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Rat serum, plasma or cell culture supernatant and organizations in the natural and recombinant AGC concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat AGC monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

22209083

ncbi acc num :

AAH36445.1

ncbi mol weight :

239,235 Da

ncbi pathways :

Disease Pathway (530764); ECM Proteoglycans Pathway (833812); Endochondral Ossification Pathway (198812); Extracellular Matrix Organization Pathway (576262); Glycosaminoglycan Metabolism Pathway (645297); Keratan Sulfate Biosynthesis Pathway (645302); Keratan Sulfate Degradation Pathway (645303); Keratan Sulfate/keratin Metabolism Pathway (645301); MPS I - Hurler Syndrome Pathway (685537); MPS II - Hunter Syndrome Pathway (685538)

ncbi summary :

This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

ACAN: This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. Defects in ACAN are the cause of spondyloepiphyseal dysplasia type Kimberley (SEDK). Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy. Defects in ACAN are the cause of spondyloepimetaphyseal dysplasia aggrecan type (SEMD-ACAN). A bone disease characterized by severe short stature, macrocephaly, severe midface hypoplasia, short neck, barrel chest and brachydactyly. The radiological findings comprise long bones with generalized irregular epiphyses with widened metaphyses, especially at the knees, platyspondyly, and multiple cervical-vertebral clefts. Defects in ACAN are the cause of osteochondritis dissecans short stature and early-onset osteoarthritis (OD). It is a type of osteochondritis defined as a separation of cartilage and subchondral bone from the surrounding tissue, primarily affecting the knee, ankle and elbow joints. It is clinically characterized by multiple osteochondritic lesions in knees and/or hips and/or elbows, disproportionate short stature and early-onset osteoarthritis. Belongs to the aggrecan/versican proteoglycan family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted; Cell adhesion; Extracellular matrix. Chromosomal Location of Human Ortholog: 15q26.1. Cellular Component: extracellular matrix; lysosomal lumen; proteinaceous extracellular matrix; Golgi lumen; extracellular region. Molecular Function: protein binding; metal ion binding; extracellular matrix structural constituent; hyaluronic acid binding; carbohydrate binding. Biological Process: keratan sulfate metabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; glycosaminoglycan metabolic process; carbohydrate metabolic process; keratan sulfate biosynthetic process; pathogenesis; keratan sulfate catabolic process; cell adhesion; proteolysis; skeletal development. Disease: Spondyloepiphyseal Dysplasia, Kimberley Type; Osteochondritis Dissecans, Short Stature, And Early-onset Osteoarthritis; Spondyloepimetaphyseal Dysplasia, Aggrecan Type

size1 :

48-Strip-Wells

price1 :

290 USD

size2 :

96-Strip-Wells

price2 :

450

more info or order :

MyBioSource product webpage