ELISA/assay

Mouse Creatine Kinase (CK) ELISA kit

MBS261027

48-Strip-Wells

290 USD

ELISA Kit

Mouse Creatine Kinase (CK) ELISA kit

Creatine Kinase (CK)

creatine kinase; Creatine kinase M-type; creatine kinase M-type; creatine kinase M chain; creatine kinase, muscle; Creatine kinase M chain; M-CKCleaved into the following chain:Creatine kinase M-type, N-terminally processed

CK

CKM; CKM; CKMM; M-CK; CKMM

KCRM_HUMAN

Mouse

381

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant CK concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse CK monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

180588

AAA96609.1

43,101 Da

Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Creatine Metabolism Pathway (106192); Creatine Pathway (413359); Creatine Pathway (468234); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); Regulation Of Retinoblastoma Protein Pathway (137916); Urea Cycle And Metabolism Of Amino Groups Pathway (198758); Creatine-phosphate Biosynthesis Pathway (545319)

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008]

CKM: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family. Protein type: Kinase, other; EC 2.7.3.2; Amino Acid Metabolism - arginine and proline. Chromosomal Location of Human Ortholog: 19q13.32. Cellular Component: cytosol. Molecular Function: creatine kinase activity; ATP binding. Biological Process: phosphocreatine biosynthetic process; phosphorylation; creatine metabolic process

48-Strip-Wells

290 USD

96-Strip-Wells

450