Bovine Neutrophil Gelatinase Associated Lipocalin (NGAL) ELISA Kit | MyBioSource MBS2609560 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Bovine Neutrophil Gelatinase Associated Lipocalin (NGAL) ELISA Kit

catalog :

MBS2609560

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2609560

products type :

ELISA Kit

products full name :

Bovine Neutrophil Gelatinase Associated Lipocalin (NGAL) ELISA Kit

products short name :

[Neutrophil Gelatinase Associated Lipocalin (NGAL)]

other names :

[neutrophil gelatinase-associated lipocalin; Neutrophil gelatinase-associated lipocalin; neutrophil gelatinase-associated lipocalin; lipocalin 2; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Oncogene 24p3; Siderocalin LCN2; p25]

products gene name :

[NGAL]

other gene names :

[LCN2; LCN2; p25; 24p3; MSFI; NGAL; HNL; NGAL; NGAL]

uniprot entry name :

NGAL_HUMAN

reactivity :

Bovine

sequence length :

198

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma or cell culture supernatant. Assay Type: Quantitative Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: Up to 0.06ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Bovine NGAL monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

38455402

ncbi acc num :

NP_005555.2

ncbi gb acc num :

NM_005564.4

ncbi mol weight :

22,457 Da

ncbi pathways :

Antimicrobial Peptides Pathway (1457777); Cytokine Signaling In Immune System Pathway (1269310); Immune System Pathway (1269170); Innate Immune System Pathway (1269203); Interleukin-4 And 13 Signaling Pathway (1470923); Iron Uptake And Transport Pathway (1269948); Metal Sequestration By Antimicrobial Proteins Pathway (1457778); Neutrophil Degranulation Pathway (1457780); Signaling By Interleukins Pathway (1269318); Transmembrane Transport Of Small Molecules Pathway (1269903)

ncbi summary :

This gene encodes a protein that belongs to the lipocalin family. Members of this family transport small hydrophobic molecules such as lipids, steroid hormones and retinoids. The protein encoded by this gene is a neutrophil gelatinase-associated lipocalin and plays a role in innate immunity by limiting bacterial growth as a result of sequestering iron-containing siderophores. The presence of this protein in blood and urine is an early biomarker of acute kidney injury. This protein is thought to be be involved in multiple cellular processes, including maintenance of skin homeostasis, and suppression of invasiveness and metastasis. Mice lacking this gene are more susceptible to bacterial infection than wild type mice. [provided by RefSeq, Sep 2015]

uniprot summary :

LCN2: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Belongs to the calycin superfamily. Lipocalin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 9q34. Cellular Component: extracellular region; extracellular space. Molecular Function: iron ion binding. Biological Process: cellular iron ion homeostasis; innate immune response; siderophore transport

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

435

size3 :

5x96-Strip-Wells

price3 :

1755

size4 :

10x96-Strip-Wells

price4 :

3150

more info or order :

MyBioSource product webpage