ELISA/assay

Human Podocalyxin (PCX) ELISA Kit

MBS260869

48-Strip-Wells

275 USD

ELISA Kit

Human Podocalyxin (PCX) ELISA Kit

Podocalyxin (PCX)

podocalyxin isoform 1; Podocalyxin; podocalyxin; GCTM-2 antigen; podocalyxin-like protein 1; podocalyxin-like; GCTM-2 antigen; Gp200; Podocalyxin-like protein 1; PC; PCLP-1

PCX

PODXL; PODXL; PC; PCLP; Gp200; PCLP-1; PCLP; PCLP1; PC; PCLP-1

PODXL_HUMAN

Human

558

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PCX concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human PCX monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

66277202

NP_001018121.1

NM_001018111.2

55,386 Da

This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008]

PODXL: Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell- cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR- dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. Belongs to the podocalyxin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 7q32-q33. Cellular Component: ruffle; extracellular space; lamellipodium; integral to plasma membrane; microvillus membrane; apical plasma membrane; cytoplasm; plasma membrane; filopodium; lipid raft. Molecular Function: protein binding. Biological Process: cell migration; negative regulation of cell-cell adhesion; negative regulation of cell adhesion; cell adhesion; positive regulation of cell-cell adhesion mediated by integrin; leukocyte migration; positive regulation of cell migration; regulation of microvillus biogenesis

48-Strip-Wells

275 USD

96-Strip-Wells

420