catalog number :
MBS260803
products type :
ELISA Kit
products full name :
Mouse Chromogranin A (CHGA) ELISA Kit
products short name :
Chromogranin A (CHGA)
other names :
chromogranin A; Chromogranin-A; Pituitary secretory protein I; SP-I
products gene name :
CHGA
other gene names :
CHGA; CgA; SP-I
uniprot entry name :
CMGA_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Mouse serum, plasma or cell culture supernatant and organizations in the natural and recombinant CHGA concentration. Assay Type: Sandwich. Detection Range: 2000 pg/ml-31.2 pg/ml. Sensitivity: 12 pg/ml
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse CHGA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
AAA52017.1
ncbi mol weight :
50,688 Da
uniprot summary :
CHGA: a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells and is a precursor to multiple biologically active peptides including vasostatin, pancreastatin, and chromostatin. Binds calcium with a low-affinity. Vasostatin negatively regulates angiogenesis and has antibacterial activity against some Gram-positive bacteria. Pancreastatin inhibits glucose induced insulin release from the pancreas. Chromostatin inhibits catecholamine release from chromaffin cells. Protein type: Vesicle; Cell adhesion; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 14q32. Cellular Component: perinuclear region of cytoplasm; extracellular region; secretory granule. Biological Process: regulation of blood pressure